中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (10): 740-742.

• 研究报道 • 上一篇    下一篇

卡介菌多糖核酸对慢性荨麻疹患者外周血淋巴细胞分泌干扰素γ和白介素4的影响

刘利君1,陆洁2,孙立新3,胡晓梅1,于淑萍4,王丹1,李雪飞1,段昕所1   

  1. 1. 河北承德医学院附属医院皮肤科
    2. 承德医学院附属医院皮肤科
    3. 承德医学院附属医院中心实验室
    4. 承德医学院附属医院
  • 收稿日期:2012-08-23 修回日期:2013-03-15 出版日期:2013-10-15 发布日期:2013-10-01
  • 通讯作者: 段昕所 E-mail:duanxinsuo2002@163.com
  • 基金资助:
    2011年度中华医学会皮肤性病学分会万马药业皮肤病学研究基金资助项目

Influence of polysaccharide nucleic acid fraction of bacillus Calmette-Guerin on the secretion of interferon-γ and interleukin-4 by peripheral blood lymphocytes from patients with chronic urticaria

  • Received:2012-08-23 Revised:2013-03-15 Online:2013-10-15 Published:2013-10-01

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摘要： 目的 探讨卡介菌多糖核酸治疗慢性荨麻疹的可能机制。 方法　用不同浓度卡介菌多糖核酸处理慢性荨麻疹患者外周血淋巴细胞，并设立健康对照组。用免疫细胞化学法和酶联免疫吸附试验（ELISA）检测卡介菌多糖核酸作用96 h后淋巴细胞分泌干扰素（IFN）-γ和白介素（IL）-4的水平。　结果　慢性荨麻疹患者外周血淋巴细胞IFN-γ的表达量明显低于健康对照组（P < 0.05），经不同浓度卡介菌多糖核酸作用96 h后，慢性荨麻疹患者外周血淋巴细胞分泌IFN-γ明显增多，且随着卡介菌多糖核酸浓度的增加而逐渐接近健康对照组。慢性荨麻疹患者外周血淋巴细胞IL-4的表达量明显高于健康对照组（P < 0.05），经不同浓度卡介菌多糖核酸作用96 h后，慢性荨麻疹患者外周血淋巴细胞分泌IL-4明显减少，且随着卡介菌多糖核酸浓度的增加而逐渐接近健康对照组。 结论　卡介菌多糖核酸体外可促进慢性荨麻疹患者外周血淋巴细胞分泌IFN-γ，抑制淋巴细胞分泌IL-4，起到调节免疫的作用。

关键词: 荨麻疹, 卡介菌多糖核酸, T淋巴细胞, 干扰素Ⅱ型, 白细胞介素4

Abstract: LIU Li-jun, LU Jie, SUN Li-xin, HU Xiao-mei, YU Shu-ping, WANG Dan, LI Xue-fei, DUAN Xin-suo. Department of Dermatology, Affiliated Hospital of Chengde Medical College, Chengde 067000, Hebei, China Corresponding author: DUAN Xin-suo, Email: duanxinsuo2002@163.com 【Abstract】 Objective To investigate the possible mechanism involved in the therapeutic potential of polysaccharide nucleic acid fraction of bacillus Calmette-Guerin （BCG-PSN） for chronic urticaria. Methods Peripheral blood lymphocytes were obtained from 30 patients with chronic urticaria, and subjected to culture in vitro with the presence of various concentrations of BCG-PSN for 96 hours. Then, immunocytochemistry and enzyme-linked immunosorbent assay （ELISA） were performed to quantify the expression levels of interferon-γ （IFN-γ） and interleukin-4 （IL-4） on, and in the culture supernatant of, these cells. The peripheral blood lymphocytes from 30 health check-up examinees served as the control. Results The untreated lymphocytes from patients with chronic urticaria showed significantly lower IFN-γ expression （P < 0.05）, but significantly higher IL-4 expression （P < 0.05） compared with those from the controls. The 96-hour treatment with BCG-PSN induced an obvious increase in the level of IFN-γ, but a marked decrease in that of IL-4, in the culture supernatant of patient-derived lymphocytes, and with the increase in BCG-PSN concentration, the levels of both IFN-γ and IL-4 secreted by patient-derived lymphocytes gradually approximated to those by the control lymphocytes. Conclusion BCG-PSN may exert an immunomodulatory effect via upregulating the secretion of IFN-γ, but downregulating that of IL-4 by peripheral blood lymphocytes from patients with chronic urticaria.

Key words: BCG-PSN, IL-4