中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (4): 210-212.

• 论著 • 上一篇    下一篇

自体黑素细胞体外培养移植治疗白癜风

朱美财1, 王雅静1, 占志1, 蔡庆1, 罗卫2, 赵广2   

  1. 1. 解放军空军总医院临床实验中心, 北京, 100036;
    2. 解放军空军总医院皮肤科, 北京, 100036
  • 收稿日期:2006-05-15 出版日期:2007-04-15 发布日期:2007-04-15

Transplantation of cultured autologous melanocytes for the treatment of vitigo

ZHU Mei-cai1, WANG Ya-jing1, ZHAN zhi1, CAi Qing1, LUO Wei2, ZHAo Guang2   

  1. Center of Clinical Trial, Air Force General Hospital of Chinese PLA, Beijing 100036, China
  • Received:2006-05-15 Online:2007-04-15 Published:2007-04-15

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摘要： 目的 探讨自体黑素细胞体外培养扩增和移植治疗白癜风的疗效.方法 用负压吸疱法在白癜风患者正常皮肤区吸疱取皮,用胰蛋白酶消化皮肤制成细胞悬液,在M2黑素细胞选择性培养基中培养,每周传代1次,经4～5次传代,以免疫荧光和多巴胺染色检测培养细胞的纯度和生物学特性.观察培养的自体黑素细胞移植到白癜风患者皮损区后色素生成效果.结果 黑素细胞能在培养基中选择性生长,经4～5周培养,获得纯的黑素细胞,无成纤维细胞和角质形成细胞污染.16例患者28个皮损区接受了移植治疗,病变皮肤面积从2cm2到200 cm2,1个月后移植区出现部分色素,呈淡红色.所有皮损区在移植后3～5个月均有色素再生,色素水平较周围正常皮肤略深或稍浅,6～8个月时色素恢复稳定,颜色与临近正常皮肤接近.87.5%皮损区色素再生面积超过50%.未见瘢痕和其他不良反应.结论 自体黑素细胞体外培养移植治疗白癜风具有效果好、安全、取较小表皮可以治疗较大面积皮损区的优点.

关键词: 过敏原, 点刺试验, 阿罗格, 过敏性疾病, 白癜风, 移植,自体, 黑素细胞

Abstract: Objective To establish a method for in vitro culture and proliferation of autologous melanocytes,and to observe the therapeutic effect of transplantation of cultured autologous melanocytes on vitiligo.Methods Blisters were induced in vitiligo patients by negative pressure suction.The roof of the blisters was clipped and digested with trypsin,then,the suspension of epidermal cells with melanocytes was selectively cultured and proliferated in serum-free M2 medium.The cells were passaged every week,and after 4-5 passages the purity and biological features of the melanocytes were assessed by immunotluorescence and DOPA staining.Then,the autologous melanocytes were transplanted onto the depigmentation areas of the vitiligo patients.Repigmentation was observed continuously in the transplanted areas.Results Melonacytes could be selectively proliferated in M2 medium.After 4-5 weeks of culture,pure melanocytes were harvested without the contamination of fibroblasts or keratinocytes.Totally,16 vitiligo patients with 28 depigmented areas(size ranging from 2 cm2 to 200 cm2) were treated with transplantation of autologous melanocytes.After one month,partial repigmentation was observed in the transplanted areas,and the skin there appeared in light red.After 3-5 months,repigmentation was observed in all the treated areas,while the color of these areas was deeper or lighter than that of the normal skin around.After 6-8 months,the pigmentation leveled off,and these areas were similar in color to the normal skin around.In addition,87.5% lesions experienced more than 50% repigmentation at this time.No scarring or other side effects were observed.Conclusion Transplantation of cultured autologous melanocytes is an effective and safe modality for the treatment of vitiligo,with the advantage of treating large depigmented area with small size of donor epidermis.

Key words: Vitiligo, Transplantation,autologous, Melanocytes