中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (9): 568-570.

• 论著 • 上一篇    下一篇

β1,4-半乳糖基转移酶-Ⅰ在瘢痕疙瘩中的表达

杨圣菊1, 陈晓栋1, 王建力1, 沈爱国2   

  1. 1. 南通大学附属医院皮肤性病科, 江苏省 226001;
    2. 南通大学微生物与免疫学教研室
  • 收稿日期:2006-09-18 出版日期:2007-09-15 发布日期:2007-09-15
  • 通讯作者: 王建力, email:pfkwang@163.com E-mail:pfkwang@163.com

Expression of β-1,4-galactosyltransferase-Ⅰin keloid tissue

YANG Sheng-ju1, CHEN Xiao-dong1, WANG Jian-li1, SHEN Ai-guo2   

  1. Department of Dermatology and Venereology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China
  • Received:2006-09-18 Online:2007-09-15 Published:2007-09-15

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摘要： 目的探讨 β1,4-半乳糖基转移酶-Ⅰ(beta-1,4-galactosyltransferase-Ⅰ, β-1,4-GalT-Ⅰ)在瘢痕疙瘩发病机制中的作用.方法取瘢痕疙瘩组织标本10份,同时以正常人皮肤6份、增生性瘢痕7份做对照,测定组织中羟脯氨酸含量,通过逆转录-聚合酶链反应(RT-PCR)和图像定量分析,检测组织中 β-1,4-GalT-Ⅰ的表达水平.结果瘢痕疙瘩组织中羟脯氨酸含量明显高于正常人皮肤组织(P<0.01). β-1,4-GalT-ⅠmRNA指数值在瘢痕疙瘩、正常人皮肤及增生性瘢痕组织分别为(0.2995±0.0825),(0.0451±0.0287),(0.2769±0.0975),瘢痕疙瘩组织与正常人皮肤比较,差异有统计学意义(P<0.01),与增生性瘢痕比较差异无统计学意义(P>0.05).瘢痕疙瘩与增生性瘢痕组织中 β-1,4-GalT-Ⅰ表达的高低与其中胶原的含量均呈正相关(r=0.9496,P<0.01;r=0.8202,P<0.05).结论 β-1,4-GalT-Ⅰ在瘢痕疙瘩纤维化进程中可能起着调节作用.

关键词: 瘢痕疙瘩, 瘢痕, 肥大性, β-N-乙酰基葡糖胺葡糖肽 β-1, 4-半乳糖转移酶

Abstract: Objective To investigate the role of β-1,4-galactosyltransferase-Ⅰ( β-1,4-GalT-Ⅰ) in the pathogenesis of keloid.Methods Tissue samples were resected from 10 patients with keloid,6 normal human controls and 7 patients with hypertrophic scar.The content of hydroxyproline was determined by alkali hydrolyzation method,and the expression of β-1,4-GalT-Ⅰgene by reverse transcription-PCR and image analysis technique.Results The content of hydroxyproline and mRNA expression of β-1,4-GalT-Ⅰwere significantly higher in the keloid tissues than in the normal controls(10.98±1.55μg/mg vs 5.38±0.47μg/mg, 0.2995±0.0825 vs 0.0451±0.0287,respectively,both P<0.01).However,no significant difference was found in either of hydroxyproline content or β-1,4-GalT-ⅠmRNA expression between tissue samples of keloid and hypertrophic scar(both P>0.05).There was a positive correlation between the mRNA expression of β-1,4-GalT-Ⅰand hydroxyproline content in the tissue of keloid(r=0.9496,P<0.01)and of hypertrophic scar.Conclusion β-1,4-GalT-Ⅰmay play a modulatory role in the fibrotic process of keloid tissue.

Key words: Keloid, Cicatrix,hypertrophic, beta-N-acetylglucosaminylglycopeptide beta-1,4-galac-tosyltransferase