中华皮肤科杂志 ›› 1993, Vol. 26 ›› Issue (1): 8-9.

• 论著 • 上一篇    下一篇

尖锐湿疣与人乳头瘤病毒DNA

  

  1. 1. 上海医科大学预防医学研究所, 244432;
    2. 上海市皮肤病防治研究所;
    3. 美国疾病控制中心
  • 收稿日期:1991-10-24 修回日期:1992-05-09 出版日期:1993-02-15 发布日期:1993-02-15

Detection of Human Papillomavirus DNA in Coeadyloma Acuminata

OU-YANG Pei-Ying, SU Jin-Ze   

  1. Institute of Prevenlive Medicine, Shanghai Medical University, 200032
  • Received:1991-10-24 Revised:1992-05-09 Online:1993-02-15 Published:1993-02-15

PDF (PC)

225

赞

摘要： 用32P标记的人乳头瘤病毒(HPV)核酸探针杂交方法检测了27例尖锐湿疣病人活检材料,25例检出HPV DNA(92.6%),均属HPV6/11型,一例伴有16/18型混合感染,另一例伴有16/18和33/33/35型混合感染.取2例用杂交方法未检出HPV DNA的活检材料提取DNA,用多聚酶链式反应(PCR)便基因扩增,再进行检测仍未检出.说明用核酸杂交方法检测的敏感性及特异性均佳,可以普遍应用于一般HPV感染的检测.

关键词: 尖锐湿疣, 人乳头瘤病毒

Abstract: Dot-Blot hybridization with 32P-labeled RNA probes was used Eo detect the presence of HPV DNA in biopsies taken from the condyloma acuminata tissue of 27 patients. HPV DNA was found in 25 cases(92.6%), all belonged to HPV 6/11.

Key words: Condyloma, acummata, Human, papillomavirus