中华皮肤科杂志 ›› 1989, Vol. 22 ›› Issue (6): 375-377.

• 论著 • 上一篇    下一篇

单克隆抗体对斑秃皮损中T细胞亚群的免疫组化表现型鉴定

侯朋, 赵永铿, 叶承耀, 黄小莹, 苏虹   

  1. 广州暨南大学医学院附属医院皮肤科
  • 收稿日期:1988-04-05 修回日期:1988-09-28 出版日期:1989-12-15 发布日期:1989-12-15

ALOPECIA AREATA; THE DEMONSTRATION OF INiMUNOHISTOCHEMICAL PHENOTYPE ON T CELL SiTIl5ET INFILTRATES WITH APAAP TECHNIQUE

HOU Peng, ZHAO Yong-Keng   

  1. Medical College, Jinan University, Guangzhou
  • Received:1988-04-05 Revised:1988-09-28 Online:1989-12-15 Published:1989-12-15

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摘要： 本文采用碱性磷酸酶-抗碱性确酸酶技术(APAAP)等方法对9例斑秃患者进行皮损中T细胞亚群鉴定,对其中7例作了毛囊部位免疫复合物沉积的检查.结果为毛囊周围主要为辅助性T细胞(TH),少量抑制性T细胞(TS).TH占62,4%.TS占11.8%,TH:TS为2.8-18.6.毛囊及其他部位均无IgG,IgM荧光标记沉积.同时发现本试验大部分病人皮损中毛囊内有大量TH,标记阳性细胞进入.

Abstract: Specimens of skin lesion on the scalp were taken from 9 untreated alopecia areata (AA) and areata totalis patients by punch biopsy.T cell subsets of 9 patients were identified by APAAp technique (OKT4,8,11) and indirect immunoperoxidase technique (Leu 2a,3a,4);and the deposition of immune complex in hair follicle was examined by direct immunofluorescence technique in 7 patients.The results were as follows: (1) 70% cellular infiltrates around hair follicles were T cells,pan T (OKT11+).Most of them were Thelper (OKT4+).62.4%;the other were T-suppresser (OKT8+),11.8%.(2) The OKT4: OKT8 ratio was about 2.8-18.6.(3) In 6 of 9 patients there were many OKT11+ and/or OKT4+ positive cells migrated into the hair follicles.It is the authous' opinion that the abnormal T cells play an important role in AA.