中华皮肤科杂志 ›› 1989, Vol. 22 ›› Issue (2): 81-82.

• 论著 • 上一篇    下一篇

用葡萄球菌A蛋白增强的念珠菌因子抗体鉴定人体念珠菌

徐参1, 于莉莉1, 陈锡唐1, 占梦松2, 贾润馥2   

  1. 1. 北京中日友好医院皮肤科;
    2. 中国人民解放军三○三医院检验科
  • 收稿日期:1987-10-29 修回日期:1988-04-18 出版日期:1989-04-15 发布日期:1989-04-15

IDENTIFICATION OF HUMAN CANDIDA BY THE SPA-ENHANCED MONOSPECIFIC ANTIBODIES OF CANDIDA

XU Can1, YU Li-Li1   

  1. Friendship Hospital, Beijing
  • Received:1987-10-29 Revised:1988-04-18 Online:1989-04-15 Published:1989-04-15

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摘要： 用葡萄球菌A蛋白菌体试剂(SPA)结合“CANDIDA CHECK”试剂盒中因子抗体,并作100倍稀释,与218株人体念珠菌作凝集试验,血清法对非血洁法的总符合率为87.2%.常见菌种白念珠菌和热带念珠菌的符合率为97.1%和98.7%少见菌种的符合率较低.此外,仅检出白念珠菌血清B型1株,未发现类星形念珠菌.本文表明采用SPA协同凝集法可以很大地节约原因子抗体量,血清法与非血清法结合可显著提高鉴定率.

Abstract: Combination of SPA with all monospecific antibodies in the kit of "CANDIDA CHECK" to enhance their sglutination titers was used in this study for the identification of Candida.Each momospecific aniibody was diluted 100 times after the combination,and then reacted with 218 strains of human Candida with slide agglutination.The total coincidence rate between serological method and nonserologieal method was 87.2%.For the common species,C.albicans and C.tropicaais,the rates were 97.1% and 98.7% respectively,and for the uncommmon species,the races were less than 60%.Combination of SPA with all monospecific antibodies in the kit of "CANDIDA CHECK" to enhance their agglutination titers was used in this study for the identification of Candida.Each momospecific aniibody was diluted 100 times after the combination,and then reacted with 218 strains of human Candida with slide agglutination.The total coincidence rate between serological method and nonserologieal method was 87.2%.For the common species,C.albicans and C.tropicaais,the rates were 97.1% and 98.7% respectively,and for the uncommmon species,the races were less than 60%.Besides,only one strain ef sero-type B of C.albicans,but none of C.tropicalis,was discovered.The results suggest that by utilizing SPA co-agglutination method,the co:aumption of original monospecific antibodies can be greatly decreased and by cooperation kof non serological method with serological method,the rate of determination can be significantly increased.