中华皮肤科杂志 ›› 1988, Vol. 21 ›› Issue (2): 70-72.

• 论著 • 上一篇    下一篇

用APAAP免疫组化方法研究CD1(OKT6)抗原在正常及SLE皮疹组织中的分布

陈辉树1, 甘芾1, 梁晓岚1, 陈璋2, 沈德诚2, 白金芬2, 边天羽3, 丁素先3, 纪华安3, 梁健3   

  1. 1. 中国医学科学院血液学研究所病理室;
    2. 中国医学科学院血液学研究所免疫室
  • 收稿日期:1987-02-05 修回日期:1987-11-13 出版日期:1988-04-15 发布日期:1988-04-15

STUDY OF THE DISTRIBUTION OF RELEVANT ANTIGEN IN TISSUES FROM NORIIZALS AND PATIENTS AND IN SLE SKIN LESIONS BY HIT6-I MONOCLONAL ANTIBODIES WITH APAAP IMMUNOHISTOCHEMICAL METHOD

Cheu Huishu1, Gan Fei1   

  1. Institute of Hematology, Chinese Academy of Medical Sicences, Tianjin
  • Received:1987-02-05 Revised:1987-11-13 Online:1988-04-15 Published:1988-04-15

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摘要： 本文以APAAP免疫组化方法及HIT6-1McAb检测了各种正常及病变组织中相应抗原的分布,所检测的正常组织包括5-6个月引产胎儿之心、肺、肾、脑、肝、平滑肌、横纹肌、甲状腺、胸腺、胃肠,成人肝、肾、扁桃体、淋巴结、骨髓等.病变组织包括ITP脾、溶血性贫血脾、再障扁桃体与食道,肺的鳞癌、腺癌及12例活动期的SLE皮疹组织.发现95以上的胸腺皮质淋巴样细胞以及扁桃体、淋巴结及脾脏中的指状突网状细胞和表皮棘细胞间LC和真皮浅层树突状网状细胞可表达HIT6-1抗原,并证实HIT6-1是CD1样McAb,并以此种McAb检测了12例SLE皮疹中LC,认为SLE皮疹中LC数目较正常者减少,而且形态也有异常.

Abstract: The distribution of relevant antigen in normal tissues and in pathological lesions were studied by HIT6_1 monoclonal antibodies with APAAP immunohistochemical method.The tissues used for assay including heart,smooth and skeletal muscle,liver,lung,kidney,brain, thyroid gland,thymus,GI tract of fetus,and liver,kindey,tonsil,lymph node and bone marrow of normal adult and spleen of patients with ITP and hemolytic anemia,tonsil of patients with aplastic anemia,cancer of esophagus and lung,and tissues of 12 cases of SLE in active stage.Over 95% lymphoid cells in thymus torttex,interdigitating cells in interfollicular area of tonsil,paracortex of lymph node,and red pulp of spleen,and Langerhans cells in the epidermis and supperfieial layer of dermis could express HIT6_1 antigen.Our result indicates that HIT6_1 is a CD1(OKT6)-like monoclonal antibody.It was also proved that the number of Langerhans cells in the skin lesions of SLE significantly reduced than that of normal,their morpholog were also abnormal.