汉防己甲素对氟康唑抗白念珠菌活性的增效作用

中华皮肤科杂志

汉防己甲素对氟康唑抗白念珠菌活性的增效作用

李丰霞, 张宏

暨南大学附属第一医院皮肤科广东药学院附属第一医院皮肤科广州 510080

收稿日期:2005-06-13 出版日期:2006-04-15 发布日期:2006-04-15
基金资助:
广东省科技计划项目(2004B33101004)

In vitro study of the synergistic effect of tetrandrine and fluconazole against Candida albicans

LI Feng-xia, ZHANG Hong

Department of Dermatology, First Affiliated Hospital of Jinan University, Guangzhou 510632, China

Received:2005-06-13 Online:2006-04-15 Published:2006-04-15

摘要/Abstract

摘要： 目的探讨体外汉防己甲素对氟康唑抗白念珠菌活性是否有增效作用.方法参照微量稀释法确定汉防己甲素对白念珠菌的非细胞毒性剂量,并测定氟康唑单独及联合汉防己甲素时对16株白念珠菌的MIC.结果在终质量浓度≤40μg/ml汉防己甲素的作用下,白念珠菌的存活率>95%.其中汉防己甲素(30～40μg/mL)可抑制白念珠菌菌丝相形成.氟康唑单独作用时,对16株白念珠菌的MIC值为0.250～64μg/mL;与汉防己甲素(40μg/mL)联合时,氟康唑对受试菌的MIC值降至0.125～16μg/mL,且终点清晰,“拖尾现象”消火.结论汉防己甲素在体外对氟康唑的抗白念珠菌活性有增效作用.

关键词: 氟康唑, 粉防己碱, 念珠菌, 白色, 微生物敏感性试验

Abstract: Objective To investigate the synergistic effect of tetrandrine and fluconazole on the growth of fluconazole-sensitive and-resistant Candida albicans in vitro.Methods The minimum concentration at which tetrandrine had no cytotoxic effect on C.albicans was determined by microdilution test.The minimum inhibitory concentration(MIC)of fluconazole alone and in combination with tetrandrine was determined against 16 strains of C.albicans by NCCLS M27-A microdilution method.Results When the concentration of tetrandrine was≤40μg/mL,the survival rate of C.albicans was greater than 95%.Tetrandrine (30～40μg/mL)inhibited the C.albicans strains from forming hyphae.The MICs of fluconazole alone to the above strains were 0.250～64μg/mL,while those of fluconazole in combination with tetrandrine (40μg/mL)were 0.125～16μg/mL,and the so-called“trailing”phenomenon disappeared.Conclusion Tetrandrine acts synergistically with fluconazole in the activity against both fluconazole-sensitive and-resistant C.albicans in vitro.

Key words: Fluconazole, Tetrandrine, Candida albicans, Microbial sensitivity tests

李丰霞, 张宏. 汉防己甲素对氟康唑抗白念珠菌活性的增效作用[J]. 中华皮肤科杂志, 2006, 39(8): 454-456.

LI Feng-xia, ZHANG Hong. In vitro study of the synergistic effect of tetrandrine and fluconazole against Candida albicans[J]. Chinese Journal of Dermatology, 2006, 39(8): 454-456.

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