中华皮肤科杂志

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18味中药乙醇提取物对小鼠B16黑素瘤细胞增殖及黑素生成的影响

涂彩霞1, 张荣鑫1, 刘亚玲3, 于志红2, 张蕴颖1, 张新军1, 刘影1   

  1. 1.大连医科大学附属第二医院皮肤科 辽宁 116027;
    2.大连医科大学附属第二医院中心实验室 辽宁 116027;
    3.大连第五人民医院皮肤科
  • 收稿日期:2005-11-08 出版日期:2006-04-15 发布日期:2006-04-15
  • 基金资助:
    辽宁省自然基金课题资助(972290)

Effects of ethanol extracts from 18 traditional Chinese herbal drugs on the proliferation of and melanogenesis in cultured B16 melanoma cells

TU Cai-xia1, ZHANG Rong-xin1, LIU Ya-ling3, YU Zhi-hong2, ZHANG Yun-ying1, ZHANG Xin-jun1, LIU Ying1   

  1. Department of Dermatology, Second Affiliated Hospital, Dalian Medical University, Dalian 116027, China
  • Received:2005-11-08 Online:2006-04-15 Published:2006-04-15

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摘要： 目的研究中药乙醇提取物对小鼠B16黑素瘤细胞增殖及黑素合成的影响。方法以MTT法测定培养的B16黑素瘤细胞的增殖活性:NaOH法测定黑素生成量;体外氧化多巴反应方法测定酪氨酸酶活性。结果与空白对照组相比,18种中药乙醇提取物中,有9种提取物对1种以上指标有显著性作用(P<0.05或P<0.01)。沙苑子、甘草、山慈姑、薄荷、苦参、夏枯草、黄芩、黑芝麻、墨旱莲可明显促进B16黑素瘤细胞增殖,甘草、山慈姑、薄荷、苦参、夏枯草、何首乌、黑芝麻、墨旱莲明显增加黑素含量,山慈姑、薄荷、苦参、夏枯草、黑芝麻、墨旱莲明显增加酪氨酸酶活性,山慈姑、薄荷、苦参、夏枯草、黑芝麻和墨旱莲的提取物对3种指标均有显著性作用(P<0.05或P<0.01)。结论山慈姑等6种中药的乙醇提取物具有促进黑素细胞增殖和合成黑素的作用。

关键词: 中草药, 黑色素瘤, 实验性, 细胞增殖, 一元酚单氧酶, 黑素合成

Abstract: Objective To study the effects of ethanol extracts from traditional Chinese herbal drugs on the proliferation of and melanogenesis in cultured B16 melanoma cells.Methods The cell proliferation of,melanogenesis and tyrosinase activity in cultured B16 cells were measured by MTT method,NaOH method and oxidative DOPA reaction,respectively.Results In the 18 tested ethanol extracts,9 gave positive Results at least in one of the three trials.As a result,Semen Astragali complanati,Radix Glycyrrhizae,Pseudobulbus Cremastrae seu Pleiones,Herba Menthae,Radix Sophorae Flavescentis,Spica Prunellae,Radix Scutellatiae,Semen Sesami Nigrum and Herba Ecliptae significantly promoted B 16 cell proliferation (P<0.05 or P<0.01);Radix Glycyrrhizae,Pseudobulbus Cremastrae seu Pleiones,Herba Menthae,Radix Sophorae Flavescentis,Spica Prunellae,Radix Polygoni Multiflori,Semen Sesami Nigrum and Herba Ecliptae significantly increased the melanogenesis in cultured B16 melanoma cells,Pseudobulbus Cremastrae seu Pleiones,Herba Menthae,Radix Sophorae Flavescentis,Spica Prunellae,Semen Sesami Nigrum and Herba Ecliptae significantly increased the activity of tyrosinase.Six ethanol extracts showed significant effects on the cell proliferation of,melanogenesis or tyrosinase activity in cultured B16 melanoma cells in all the trials (P<0.05 or P<0.01).Conclusions Ethanol extracts from the 6 traditional Chinese herbal drugs (Pseudobulbus Cremastrae seu Pleiones,Herba Menthae,Radix Sophorae Flavescentis,Spica Prunellae,Semen Sesami Nigrum and Herba Ecliptae) can promote the proliferation of and melanogenesis in cultured B16 cells.

Key words: Drugs,Chinese herbal, Melanoma,experimental, Cell proliferation, Monophenol monooxygenase, Melanogenesis