中华皮肤科杂志

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苯甲酸雌二醇及UVA辐照对体外培养的人皮肤成纤维细胞胶原合成的影响

李晓凤, 范卫新, 张卉   

  1. 南京医科大学第一附属医院皮肤科 2010029
  • 收稿日期:2005-11-11 出版日期:2006-04-15 发布日期:2006-04-15
  • 基金资助:
    国家自然科学基金(30170860)

Effects of estradiol benzoate and UVA radiation on collagen synthesis in cultured human fibroblasts

LI Xiao-feng, FAN Wei-xin, ZHANG Hui   

  1. Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
  • Received:2005-11-11 Online:2006-04-15 Published:2006-04-15

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摘要： 目的探讨苯甲酸雌二醇对体外培养的人成纤维细胞增殖、胶原合成及其对UVA抑制胶原合成的影响。方法在培养的人成纤维细胞中分别加入不同剂量的苯甲酸雌二醇,继续培养48h,用MTT法测定细胞的增殖情况,同时应用RT-PCR方法检测经不同剂量苯甲酸雌二醇及10J/cm2UVA处理后人成纤维细胞Ⅰ、Ⅲ型前胶原mRNA的表达情况。结果MTT法检测结果显示,苯甲酸雌二醇对体外培养的成纤维细胞增殖无明显促进作用。RT-PCR结果提示,苯甲酸雌二醇处理组两型前胶原的表达水平明显上调(P<0.05),而UVA照射后两型前胶原的表达显著下降,苯甲酸雌二醇可在一定水平对抗其作用(P<0.05)。结论苯甲酸雌二醇对体外培养的成纤维细胞Ⅰ、Ⅲ型前胶原的合成有一定的促进作用,并可对抗紫外线抑制胶原合成的作用。

关键词: 雌二醇, 紫外线, 成纤维细胞, 胶原Ⅰ型, 胶原Ⅲ型

Abstract: Objective To explore the effects of estradiol benzoate on the proliferation of collagen synthesis and UVA-induced inhibition of collagen synthesis in cultured human skin fibroblasts.Methods The human skin fibroblasts were cultured in various concentrations (2,4,8,16,32,64,128 nmol/L) of estradiol benzoate in vitro.MTT method was used to assess the proliferation of the cells.To investigate the effects of estradiol benzoate on the UVA-induced inhibition of collagen synthesis,the cultured fibroblasts were divided into four groups:blank control,treatment with estradiol benzoate (2,4,8,16 nmol/L,respectively) only,treatment with UVA radiation (10 J/cm2)only,and treatment with both estradioi benzoate and UVA radiation (4 nmol/L estradiol benzoate + 10 J/cm2 UVA and 16 nmol/L estradiol benzoate + 10 J/cm2 UVA,respectively).After 48 h of culture,total RNA was extracted from the cells,then the mRNA expression of type I andⅢprecollagens was examined by RT-PCR.Results MTT assay showed that estradiol benzoate had no effect on the proliferation of fibroblasts in vitro.The mRNA expression of the two types of precollagen was up-regulated in the group treated with estradiol benzoate only.On the contrary,the synthesis of the two types of precoilagen was significantly inhibited by UVA radiation,while estradiol benzoate could block the inhibition (P<0.05).Conclusion Estradiol benzoate can improve the synthesis of typeⅠandⅢ precollagens in cultured human skin fibroblasts,and block the inhibitory effect of UVA radiation on the precollagen synthesis.

Key words: Estradiol, Ultraviolet rays, Fibroblasts, Collagen typeⅠ, Collagen typeⅢ