中华皮肤科杂志

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中国南方部分城市泌尿生殖道沙眼衣原体基因分型研究

陈丽丽1, 吴移谋1, 邓仲良1, 蔡恒玲1, 余敏君1, 顾伟民2, 张俊芳3   

  1. 1. 南华大学病原生物学研究所 湖南衡阳 421001;
    2. 上海市皮肤病性病医院;
    3. 南华大学第二附属医院
  • 收稿日期:2005-08-31 出版日期:2006-04-15 发布日期:2006-04-15
  • 通讯作者: 吴移谋,email:yimouwu@sina.com E-mail:email:yimouwu@sina.com
  • 基金资助:
    湖南省科技厅重点项目基金(04SK2003)

Genotyping of Chlamydia trachomatis isolated from patients with sexually transmitted diseases from four cities in Southern China

CHEN Li-li1, WU Yi-mou1, DENG Zhong-liang1, CAI Heng-ling1, YU Min-jun1, GU Wei-min2, ZHANG Jun-fang3   

  1. Institute of Pathogenic Biology, Nanhua University, Hengvang 421001, Hunan, China
  • Received:2005-08-31 Online:2006-04-15 Published:2006-04-15

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摘要： 目的 了解中国南方4个城市性病高危人群中沙眼衣原体(Ct)基因型的分布特征。方法 从衡阳、上海、广州、江门四个城市的性病门诊收集1180份泌尿生殖道标本,经质粒PCR筛选后,阳性者用巢式PCR扩增Ctomp1基因片段,酶切omp1扩增产物,分析Ct各临床株omp1基因的限制性片段长度多态性(RFLP),将临床株分型。结果 Ct质粒PCR阳性者301份,用于分型者286份。通过RFLP分型,共检出10个基因型,其中常见的是E(30.8%)、J(23.8%)、D(17.5%)、F(10.5%)、G(6.2%)型,并且不同城市间基因型的分布差异无统计学意义(χ2=0.981,P>0.05)。结论 4个城市存在Ct多种基因型,并且基因型的分布大致相同。

关键词: 衣原体, 沙眼, 基因, ompl, 多态性, 限制性片段长度

Abstract: Objective To investigate the distribution of Chlamydia trachomatis genotypes in high risk populations of STDs from four cities in Southern China.Methods C.trachomatis was detected by a plasmid PCR in 1180 specimens collected in Hengyang,Shanghai,Guangzhou and Jiangmen,and then the major outer membrane protein gene (ompl) was amplified by nested PCR (nPCR) with plasmid-PCR-positive specimens.The amplicons of ompl were digested by restriction endonuclease Alu I,Msp I,EcoR I,or Dde I.C.trachoraatis was genotyped according to the restriction fragment length polymorphism (RFLP) patterns.Results Of 301 plasmid-PCR-positive specimens,286 (48 collected from Hengyang,32 from Shanghai,52 from Guangzhou and 154 from Jiangmen) could be genotyped.The most prevalent genotype was serotype E(30.8%),followed by J(23.8%),D(17.5%),F(10.5%),and G (6.2%).The distribution of various genotypes was not different among different cities.Conelusion Polymorphic genotypes of C.trachomatis are prevalent in Hengyang,Shanghai,Guangzhou and Jiangmen,and there is no significant difference of genotype distribution among these four cities.

Key words: Chlamydia trachomatis, Genes,ompl, Polymorphism,restriction fragment length