中华皮肤科杂志 ›› 1989, Vol. 22 ›› Issue (5): 302-303.

• 论著 • 上一篇    下一篇

吸引疱疱壁郎格罕细胞的实验研究

王秉鹤, 赵辫, 葛以信, 张晓军, 陈希平, 张美华   

  1. 江苏省人民医院皮肤科
  • 收稿日期:1988-03-30 修回日期:1988-08-30 出版日期:1989-10-15 发布日期:1989-10-15

Experimental study of Langerhans cells of suction blister roof

WANG Bing-He, ZHAO Bian   

  1. Jiangsu Province People's Hospital, Nanjing
  • Received:1988-03-30 Revised:1988-08-30 Online:1989-10-15 Published:1989-10-15

PDF (PC)

240

赞

摘要： 用吸引器在豚鼠皮肤负压吸引1小时左右,使之形成水疱.这种吸引疱位于表皮、真出皮连接处,疱顶是完整表皮.剪下疱顶,以ATP酶法对郎格罕细胞进行细胞化学染色,取得十分满意效果,由于取材不涉及真皮组织,水疱愈后不会遗留疤痕,因此尤其适合于面部等暴露部位的表皮郎格罕细胞检查.

Abstract: Suction blisters were raised over guinea pig skin with a suction pump. The time required for blister formation was approximately one hour. The blisters were formed in dermal-epidermal junction. Each blister top, consisting entirely of epidermis, was then removed with scissors. Specimens were processed cytochemitally for adenosine triphosphatase (ATPase).A good result was obtained. Because there was no dermis in the biopsy, no scar was formed after wound healing. It is specially suitable for the examination of epidermal Langerhana cells over exposure areas such as face.