中华皮肤科杂志 ›› 2017, Vol. 50 ›› Issue (1): 48-50.

• 研究报道 • 上一篇    下一篇

白癜风患者血清中抗酪氨酸酶IgG抗体及抗酪氨酸酶相关蛋白1 IgG抗体的检测

韩莹1,李铁男1,1,刘文力1,金春琳1,程欣1,刘奕2,贺莎1,吴善东3   

  1. 1. 沈阳市第七人民医院
    2. 杭州浙大迪迅生物基因工程有限公司
    3. 浙江大学过敏研究中心
  • 收稿日期:2016-04-13 修回日期:2016-06-18 出版日期:2017-01-15 发布日期:2017-01-05
  • 通讯作者: 李铁男 E-mail:ltn8899@126.com
  • 基金资助:
    沈阳市科技创新专项资金-人口与健康科技攻关专项

Detection of anti?tyrosinase IgG antibody and anti?tyrosinase?related protein?1 IgG antibody in sera of patients with vitiligo

  • Received:2016-04-13 Revised:2016-06-18 Online:2017-01-15 Published:2017-01-05

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摘要： 目的 探讨白癜风患者血清中抗酪氨酸酶IgG抗体（TYR IgG）和抗酪氨酸酶相关蛋白1 IgG抗体（TRP?1 IgG）与白癜风的关系。方法 用酶联免疫吸附测定检测260例白癜风患者和50例健康对照血清中TYR IgG和TRP?1 IgG的含量。结果 白癜风组TYR IgG 和（或）TRP?1 IgG阳性率57.31%（149/260），TYR IgG阳性率37.3%（97/260），TRP?1 IgG阳性率33.5%（87/260），均明显高于对照组（χ2 = 25.441、21.630，P＜0.01）。TYR IgG阳性与TRP?1 IgG阳性不相关（r = -0.032，P＞0.05）。白癜风组TYR IgG阳性率男女间差异无统计学意义（P＞0.05），但TRP?1 IgG阳性率女性高于男性（χ2 = 5.811，P＜0.05）。白癜风TYR IgG阳性率 ≤ 20岁（35.9%）与＞20岁（38.3%）比较，差异无统计学意义（P＞0.05），而TRP?1 IgG阳性率 ≤ 20岁组明显高于＞20岁组（χ2 = 6.498，P＜0.05）。结论 检测TYR IgG和TRP?1 IgG可为白癜风的诊断和治疗提供一定依据。

Abstract: Han Ying, Li Tienan, Liu Wenli, Jin Chunlin, Cheng Xin, Liu Yi, He Sha, Wu Shandong Department of Dermatology, Shenyang No.7 People′s Hospital, Shenyang 110003, China （Han Y, Li TN, Liu WL, Jin CL, Cheng X）; Hangzhou Zheda Dixun Biological Gene Engineering Co., Ltd, Hangzhou 310052, China （Liu Y, He S, Wu SD） Corresponding author: Li Tienan, Email: qiyuanmys@163.com 【Abstract】 Objective To investigate relationships between serum levels of anti?tyrosinase IgG antibody （TYR IgG） as well as anti?tyrosinase?related protein?1 IgG antibody （TRP?1 IgG） and vitiligo. Methods Enzyme linked immunosorbent assay （ELISA） was performed to detect serum levels of TYR IgG and TRP?1 IgG in 260 patients with vitiligo and 50 health controls. The threshold for defining a positive test result was set at 3 standard deviations above the mean serum level of TYR IgG or TRP?1 IgG in the healthy controls. Results The positive rate of TYR IgG and/or TRP?1 IgG in the vitiligo group was 57.31% （149/260）. The positive rates of TYR IgG and TRP?1 IgG were both significantly higher in the vitiligo group than in the control group （TYR IgG: 37.3%［97/260］ vs. 0, χ2 = 25.441, P < 0.01; TRP?1 IgG: 33.5%［87/260］ vs. 0, χ2 = 21.630, P < 0.01）. The positive rate of TYR IgG was not associated with that of TRP?1 IgG in the vitiligo group （r = -0.032, P > 0.05）. Among patients with vitiligo, the positive rate of TRP?1 IgG was significantly higher in females than in males （χ2 = 5.811, P < 0.05）, as well as in patients aged ≤ 20 years than in those aged > 20 years （χ2 = 6.498, P < 0.05）, while the positive rate of TYR IgG didn′t differ between females and males, or between patients aged ≤ 20 years and those aged > 20 years （both P > 0.05）. Conclusion Detection of TYR IgG and TRP?1 IgG may provide some evidence for the diagnosis and treatment of vitiligo.