中华皮肤科杂志 ›› 2017, Vol. 50 ›› Issue (1): 44-45.

• 研究报道 • 上一篇    下一篇

大疱性类天疱疮患者疱液中趋化因子受体5和可诱导共刺激分子的检测

姜蔚蔚1,朱英华2,杨敏2,3,岳宏元2,王红梅3,杨海琼2,焦阳1   

  1. 1. 大连医科大学，大连市皮肤病医院
    2. 大连市皮肤病医院
    3.
  • 收稿日期:2016-03-17 修回日期:2016-08-26 出版日期:2017-01-15 发布日期:2017-01-05
  • 通讯作者: 朱英华 E-mail:zyh-office@163.com

Detection of C?X?C chemokine receptor type 5 and inducible costimulator in blister fluid of patients with bullous pemphigoid

  • Received:2016-03-17 Revised:2016-08-26 Online:2017-01-15 Published:2017-01-05

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摘要： 目的 检测C?X?C趋化因子受体5（CXCR5）和可诱导共刺激分子（ICOS）在大疱性类天疱疮（BP）患者疱液中的水平，探讨其在BP发病机制中的意义。方法 收集15例BP患者疱液为实验组，15例Ⅱ度烧伤患者疱液为对照组，采用酶联免疫吸附测定（ELISA）方法分别检测CXCR5及ICOS在两组疱液中的水平。结果 实验组及对照组疱液中CXCR5水平分别为（219 ± 145.31）、（147 ± 23.83） ng/L，差异有统计学意义（t = 4.577，P < 0.05），但疱液中ICOS水平差异无统计学意义［（30.18 ± 14.86）比（21.43 ± 5.32） ng/L，t = 1.628，P > 0.05］。结论 CXCR5的表达可能与BP发病有一定关系，而ICOS与BP发病的关系尚需进一步研究。

Abstract: Jiang Weiwei, Zhu Yinghua, Yang Min, Yue Hongyuan, Wang Hongmei, Yang Haiqiong, Jiao Yang Dalian Medical University, Dalian 116021, China （Jiang WW, Jiao Y）; Department of Dermatology, Dalian Dermatosis Hospital, Dalian 116021, China （Zhu YH, Yang M, Yue HY, Wang HM, Yang HQ） Corresponding author: Zhu Yinghua, Email: zyh-office@163.com 【Abstract】 Objective To detect levels of C-X-C chemokine receptor type 5 （CXCR5） and inducible costimulator （ICOS） in blister fluid of patients with bullous pemphigoid （BP）, and to explore their significance in the pathogenesis of BP. Methods Blister fluid samples were collected from 15 patients with BP （experimental group） and 15 patients with second-degree burns （control group）. Enzyme-linked immunosorbent assay （ELISA） was performed to detect the levels of CXCR5 and ICOS in the 2 groups. Results The level of CXCR5 was significantly higher in the experimental group than in the control group （219 ± 145.31 vs. 147 ± 23.83 ng/L, t = 4.577, P < 0.05）, while no significant difference in the ICOS level was observed between the 2 groups （30.18 ± 14.86 vs. 21.43 ± 5.32 ng/L, t = 1.628, P > 0.05）. Conclusion The of CXCR5 may be associated with the occurrence of BP, but further researches are needed to determine the relationship between ICOS and the occurrence of BP.