中华皮肤科杂志 ›› 2017, Vol. 50 ›› Issue (1): 13-17.

• 论著 • 上一篇    下一篇

Toll样受体7、9及其调控分子MyD88、NF-κB在白癜风患者外周血单个核细胞中的表达

唐亚平1,盛文婷2,周欣1,黎小东1,田歆1,钟金宝1,李振洁1,林春生1,刘玉梅3   

  1. 1. 广州市皮肤病防治所
    2.
    3. 广州市皮肤病防治所皮肤科
  • 收稿日期:2016-09-23 修回日期:2016-10-17 出版日期:2017-01-15 发布日期:2017-01-05
  • 通讯作者: 唐亚平 E-mail:tangya1130@126.com
  • 基金资助:
    广东省科技计划项目;广州市医药卫生科技项目

Expression of Toll?like receptors 7 and 9 as well as their regulatory molecules myeloid differentia?tion factor 88 and nuclear factor?κB in peripheral blood mononuclear cells of patients with vitiligo

  • Received:2016-09-23 Revised:2016-10-17 Online:2017-01-15 Published:2017-01-05

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摘要： 目的 探讨Toll样受体7、9（TLR7、TLR9）及调控分子MyD88、NF?κB在白癜风患者以及健康个体外周血单个核细胞（PBMC）中的表达差异及意义。方法 用流式细胞仪检测36例白癜风患者及22例健康对照PBMC中TLR7、TLR9的表达，并用反转录聚合酶链反应（RT?PCR）检测上述样本中MyD88、NF?κB mRNA的表达。结果 白癜风患者TLR7的表达高于健康对照组，但差异无统计学意义（t = 1.477，P = 0.145）；TLR9的表达低于健康对照组，差异无统计学意义（t = -1.761，P = 0.084）。白癜风患者外周血PBMC中MyD88 mRNA的表达稍高于对照组，但差异无统计学意义（t = 0.058，P = 0.954）；NF?κB mRNA的表达明显高于对照组，差异有统计学意义（t = 2.814，P = 0.008）。结论 TLR7、TLR9的调控通路中的关键信号分子NF?κB表达升高，提示NF?κB可能参与白癜风的发病机制。

Abstract: Tang Yaping, Sheng Wenting, Zhou Xin, Li Xiaodong, Tian Xin, Zhong Jinbao, Li Zhenjie, Lin Chunsheng, Liu Yumei Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou 510095, China （Tang YP, Zhou X, Li XD, Tian X, Zhong JB, Li ZJ, Lin CS, Liu YM）; Department of Dermatology, Guangzhou Red Cross Hospital, Guangzhou 510220, China （Sheng WT） Corresponding author: Tang Yaping, Email: tangya1130@126.com 【Abstract】 Objective To compare of Toll-like receptors 7 and 9 （TLR7, TLR9） as well as their regulatory molecules myeloid differentiation factor 88 （MyD88） and nuclear factor-κB （NF-κB） in peripheral blood mononuclear cells （PBMCs） between patients with vitiligo and healthy individuals, and to explore their significance. Methods Flow cytometry was performed to measure of TLR7 and TLR9 in PBMCs among 36 patients with vitiligo and 22 healthy controls, and real-time fluorescence-based quantitative PCR （RT-PCR） was conducted to determine mRNA of MyD88 and NF-κB in the above blood samples. Results Compared with healthy controls, patients with vitiligo showed higher of TLR7 and mRNA of MyD88 and NF-κB, but lower of TLR9. However, significant differences were only observed in the mRNA of NF-κB （t = 2.814, P = 0.008）, but not in the of TLR7 and TLR9 or the mRNA of MyD88 between patients and controls （t = 1.477, 1.761, 0.058, all P > 0.05）. Conclusion NF-κB, as a key signaling molecule of TLR7 and TLR9 regulation pathways, increases obviously in patients with vitiligo, suggesting that NF-κB may be involved in the pathogenesis of vitiligo.