葛根素促进黑素细胞黑素合成及其机制的初步探讨

中华皮肤科杂志 ›› 2016, Vol. 49 ›› Issue (5): 338-341.

葛根素促进黑素细胞黑素合成及其机制的初步探讨

章玲玲¹,许文²,许爱娥³

1. 浙江中医药大学附属杭州第三医院
2. 杭州市第三人民医院
3. 安徽医科大学附属杭州市第三人民医院皮肤科

收稿日期:2015-08-27 修回日期:2015-12-03 出版日期:2016-05-15 发布日期:2016-05-04
通讯作者: 许爱娥 E-mail:xuaiehz@msn.com
基金资助:
Interlenkin 6/gp130信号通路影响白癜风移植疗效的机制;SENP2介导的SUMO化修饰在白癜风移植中的作用及干预机制;优化自体黑素细胞移植治疗白癜风的技术及应用;茶多酚中治疗白癜风的活性单体的筛选及临床前研究

Promotive effect of puerarin on melanogenesis in melanocytes and its possible mechanisms

Received:2015-08-27 Revised:2015-12-03 Online:2016-05-15 Published:2016-05-04

摘要/Abstract

摘要：

目的探讨葛根素对正常人黑素细胞黑素合成的影响和可能机制。方法用噻唑蓝（MTT）法、NaOH裂解法观察葛根素对黑素细胞增殖及黑素合成作用，RT-PCR及Western印迹法检测葛根素对黑素细胞小眼畸形相关转录因子（MITF）、酪氨酸酶（TYR）、酪氨酸酶相关蛋白1（TRP-1）基因转录和蛋白表达水平的影响。结果 1 ~ 40 μmol/L浓度范围内葛根素对体外培养的黑素细胞增殖影响与正常对照组相比，差异无统计学意义（P > 0.05）。与正常对照组相比，40 μmol/L葛根素可显著促进黑素细胞的黑素合成（P < 0.05），并可显著增加MITF、TYR、TRP-1 mRNA及蛋白的表达（P < 0.05）。40 μmol/L葛根素使MITF、TYR、TRP-1的蛋白表达量分别比正常对照组增加8.69%，10.28%和10.58%（P < 0.05）；并使MITF、TYR、TRP-1的mRNA表达量分别比正常对照组增加2.48倍，1.91倍和1.63倍（P < 0.05）。结论葛根素能增加MITF、TYR、TRP-1 mRNA及蛋白的表达水平，促进黑素合成。

Abstract:

Zhang Lingling, Xu Wen, Xu Ai′e Department of Dermatology, Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310009, China Corresponding author: Xu Ai′e, Email: xuaiehz@msn.com 【Abstract】 Objective To evaluate the effect of puerarin on melanogenesis in melanocytes, and to explore its possible mechanisms. Methods Third- to fifth-passage melanocytes isolated from human foreskin were treated with different concentrations （1, 5, 10, 20, 40, 80 and 160 μmol/L） of puerarin for 24 hours, with those receiving no treatment as the normal control group. Methyl thiazolyl tetrazolium （MTT） assay was performed to evaluate the proliferative activity of melanocytes, a sodium hydroxide solubilization method was used to measure melanin content, and reverse transcription PCR （RT-PCR） and Western blot analysis were performed to quantify the mRNA and protein s of microphthalmia-associated transcription factor （MITF）, tyrosinase （TYR） and tyrosinase-related protein-1 （TRP-1） respectively. Results There were no significant differences in the proliferative activity of melanocytes between the puerarin （1 - 40 μmol/L） groups and normal control group （P > 0.05）, and 40 μmol/L was chosen as the concentration of puerarin for subsequent experiments. Compared with the normal control group, the 40-μmol/L puerarin group showed increased melanin content as well as mRNA and protein s of MITF, TYR and TRP-1 （all P < 0.05）. Concretely speaking, the protein s of MITF, TYR and TRP-1 in the 40-μmol/L puerarin group were increased by 8.69%, 10.28% and 10.58% compared with the normal control group respectively （all P < 0.05）, and their mRNA s were 2.48, 1.91 and 1.63 times higher in the 40-μmol/L puerarin group than in the normal control group respectively （all P < 0.05）. Conclusion Puerarin can increase the mRNA and protein s of MITF, TYR and TRP-1, and promote melanogenesis in melanocytes.

章玲玲许文许爱娥. 葛根素促进黑素细胞黑素合成及其机制的初步探讨[J]. 中华皮肤科杂志, 2016, 49(5): 338-341.

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