系统性红斑狼疮患者STAT3表达水平及其与DNA甲基化相关性研究

中华皮肤科杂志 ›› 2016, Vol. 49 ›› Issue (2): 108-111.

系统性红斑狼疮患者STAT3表达水平及其与DNA甲基化相关性研究

朱小华¹,梁俊¹,杨永生¹,徐金华²

1. 复旦大学附属华山医院
2. 上海市复旦大学附属华山医院皮肤科

收稿日期:2015-04-13 修回日期:2015-09-29 出版日期:2016-02-15 发布日期:2016-02-04
通讯作者: 徐金华 E-mail:xjhhsyy@163.com,hsyyxjh@163.com
基金资助:
光敏相关性microRNA在紫外线诱发狼疮中调控DNA甲基化作用的研究;光敏相关性microRNA筛查及其在紫外线诱发狼疮中的作用机制研究

STAT3 expression and its relationship with DNA methylation in patients with systemic lupus erythematosus

Received:2015-04-13 Revised:2015-09-29 Online:2016-02-15 Published:2016-02-04
Contact: XU Jinhua E-mail:xjhhsyy@163.com,hsyyxjh@163.com

摘要/Abstract

摘要：

目的探讨系统性红斑狼疮（SLE）患者外周血T淋巴细胞DNA甲基化水平、信号传导与转录激活因子3（STAT3）基因表达水平及二者的相关性。方法取34例SLE患者和23例健康对照者外周血，分离T淋巴细胞，采用5甲基胞嘧啶抗体与流式细胞仪检测DNA甲基化状态，用反转录-聚合酶链反应（RT-PCR）分析STAT3 mRNA表达水平。结果 SLE活动期患者17例DNA甲基化水平（8.50 ± 1.42）显著低于健康对照者（11.31 ± 1.34，P < 0.01），而其STAT3基因表达水平（1.34 ± 0.32）显著高于健康对照者（1.02 ± 0.28，P < 0.01）。SLE缓解期患者17例DNA甲基化水平（11.30 ± 1.34）及STAT3基因表达水平（1.01 ± 0.27）与健康对照组相比，差异无统计学意义（均P > 0.05）。SLE活动期患者DNA甲基化水平显著低于缓解期（P < 0.01），而其STAT3基因表达水平显著高于缓解期（P < 0.01）。Pearson相关分析显示，SLE患者DNA甲基化水平与SLE疾病活动指数（SLE-DAI）呈负相关（r = -0.78，P < 0.01），STAT3基因表达水平与SLE-DAI呈正相关（r = 0.50，P < 0.01），而STAT3基因表达水平与DNA甲基化水平无相关（r = -0.13，P > 0.05）。结论 STAT3在外周血T淋巴细胞中的异常表达与SLE的病情活动相关。STAT3基因表达水平与DNA甲基化水平无相关性。

Abstract:

Zhu Xiaohua, Liang Jun, Yang Yongsheng, Xu Jinhua Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China Corresponding author: Xu Jinhua, Email: hsyyxjh@163.com 【Abstract】 Objective To investigate DNA methylation status as well as signal transducer and activator of transcription 3（STAT3） gene expression in peripheral blood T-lymphocytes from patients with systemic lupus erythematosus （SLE）, and to explore their relationship. Methods Peripheral blood samples were obtained from 34 patients with SLE and 23 healthy controls followed by the isolation of T-lymphocytes. Flow cytometry was performed to evaluate DNA methylation status using antibodies against 5-methylcytosine （5-mc）, reverse transcription （RT）-PCR to detect the mRNA expression of STAT3 in T-lymphocytes. Results Compared with healthy controls, 17 patients with active SLE showed significantly decreased DNA methylation levels （8.50 ± 1.42 vs. 11.31 ± 1.34, P < 0.01）, but increased STAT3 mRNA expression levels （1.34 ± 0.32 vs. 1.02 ± 0.28, P < 0.01）. However, there were no significant differences between 17 patients with stable SLE and healthy controls in DNA methylation levels （11.30 ± 1.34 vs. 11.31 ± 1.34, P > 0.05） or STAT3 mRNA expression levels （1.01 ± 0.27 vs. 1.02 ± 0.28, P > 0.05）. Patients with active SLE also showed significantly reduced DNA methylation levels, but enhanced STAT3 mRNA expression compared with those with stable SLE （both P < 0.01）. As Pearson correlation analysis showed, SLE disease activity index （SLE-DAI） was negatively correlated with DNA methylation levels in patients with SLE （r = － 0.78, P < 0.01）, but positively correlated with STAT3 mRNA expression （r = 0.50, P < 0.01）, and no significant correlation was observed between STAT3 mRNA expression and DNA methylation levels （r = － 0.13, P > 0.05）. Conclusion The aberrant expression of STAT3 in peripheral blood T-lymphocytes may be related to disease activity in SLE, but unrelated to DNA methylation levels.

朱小华梁俊杨永生徐金华. 系统性红斑狼疮患者STAT3表达水平及其与DNA甲基化相关性研究[J]. 中华皮肤科杂志, 2016, 49(2): 108-111.doi:

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