中华皮肤科杂志 ›› 2015, Vol. 48 ›› Issue (9): 625-628.

• 论著 • 上一篇    下一篇

活化抗原CD69、HLA-DR在寻常性银屑病外周血单一核细胞和皮损中的表达

陈文娟,顾俊瑛,龚瑜,刘至昱,许辉,熊惠姊,史玉玲   

  1. 同济大学附属第十人民医院
  • 收稿日期:2014-12-31 修回日期:2015-06-26 出版日期:2015-09-15 发布日期:2015-09-01
  • 通讯作者: 史玉玲 E-mail:shiyuling1973@gmail.com
  • 基金资助:

    IL-27对银屑病iNKT细胞和Th17细胞的免疫调节和效应应答的研究;IL-27对银屑病iNKT细胞和Th17细胞的免疫调节和效应应答的研究;IL-27对银屑病iNKT细胞和Th17细胞的免疫调节和效应应答的研究

Expressions of activation antigens CD69 and HLA-DR in peripheral blood mononuclear cells and skin lesions of patients with psoriasis vulgaris

  • Received:2014-12-31 Revised:2015-06-26 Online:2015-09-15 Published:2015-09-01
  • Contact: SHI YuLing E-mail:shiyuling1973@gmail.com

PDF

6

赞

摘要：

目的 探讨活化抗原CD69和HLA-DR在寻常性银屑病患者外周血CD3+T淋巴细胞和皮损中的表达变化。 方法 流式细胞仪检测20例寻常性银屑病患者和20例健康对照外周血CD3+T细胞中CD69和HLA-DR的表达，同时采用免疫组化的方法检测15例寻常性银屑病患者和10例健康对照皮损组织中HLA-DR的表达并进行对比。两组统计学分析采用双侧t检验。 结果 CD69和HLA-DR在寻常性银屑病患者外周血CD3+T细胞中的表达率分别为（4.70 ± 1.90）%、（8.97 ± 1.79）%，比健康对照组［（1.56 ± 0.95）%、（3.02 ± 1.15）%］明显升高（均P < 0.01）。Pearson相关性分析发现，CD3+HLA-DR+细胞百分比与银屑病皮损和严重程度评分指数（PASI）评分呈正相关（r = 0.5626，P < 0.05）。与健康对照组比较，寻常性银屑病患者皮损真皮中HLA-DR表达升高，但表皮中HLA-DR表达降低（均P < 0.05），HLA-DR在健康人主要分布在血管周围，而在寻常性银屑病患者则广泛表达于真皮组织。 结论 寻常性银屑病患者外周血CD3+T淋巴细胞及皮损中的炎症细胞存在异常活化，外周血CD3+HLA-DR+细胞与疾病严重程度存在相关性。

Abstract:

Chen Wenjuan, Gu Junying, Gong Yu, Liu Zhiyu, Xu Hui, Xiong Huizi, Shi Yuling. Department of Dermatology, Shanghai Tenth People′s Hospital of Tongji University, Shanghai 200072, China Corresponding author: Shi Yuling, Email: shiyuling1973@gongji.edu.cn 【Abstract】 Objective To investigate changes in expressions of activation antigens CD69 and HLA-DR in CD3+ T lymphocytes in peripheral blood and skin lesions in patients with psoriasis vulgaris. Methods Peripheral blood samples were obtained from 20 patients with psoriasis vulgaris and 20 healthy controls, and skin specimens from the lesions of 15 out of the 20 patients and 10 healthy controls. Flow cytometry was performed to quantify the expressions of CD69 and HLA-DR in peripheral blood CD3+ T cells, and an immunohistochemical study to measure the expression of HLA-DR in skin specimens. Statistical analysis was carried out by a two-sample t-test and Pearson correlation analysis with the SPSS 19.0 software. Results Compared with the healthy controls, the patients with psoriasis vulgaris showed increased expression rates of CD69 （4.70% ± 1.90% vs. 1.56% ± 0.95%, t = 6.629, P < 0.01） and HLA-DR （8.97% ± 1.79% vs. 3.02% ± 1.15%, t = 6.204, P < 0.01） in peripheral blood. Pearson correlation analysis revealed that the percentage of CD3+HLA-DR+ cells in peripheral blood was positively correlated with the psoriasis area and severity index （PASI） score （r = 0.5626, P < 0.05）. The expression rate of HLA-DR was significantly higher in the dermis （64.87% ± 17.31% vs. 19.80% ± 5.69%, t = 7.916, P < 0.01）, but lower in the epidermis （11.80% ± 5.55% vs. 27.40% ± 8.61%, t = 5.479, P < 0.01） in the psoriatic specimens compared with the control specimens. Immunohistochemically, HLA-DR was widely expressed in the dermis of psoriatic lesions, but mainly distributed around blood vessels in the control skin. Conclusions There is an aberrant activation of CD3+ T cells in peripheral blood and inflammatory cells in skin lesions in patients with psoriasis vulgaris, and the percentage of CD3+HLA-DR+ cells in peripheral blood is correlated with the severity of psoriasis vulagaris.