中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (9): 665-666.

• 研究报道 • 上一篇    下一篇

卡介菌多糖核酸对尖锐湿疣患者外周血CD4+CD25+Foxp3+调节性T细胞的影响

王振华1,杨芳红1,孙怡2,王辉3,薛峰4   

  1. 1. 山东省潍坊市人民医院皮肤科
    2. 潍坊市人民医院皮肤科
    3. 潍坊市人民医院 广文街151号
    4. 山东省潍坊市人民医院检验科
  • 收稿日期:2014-02-26 修回日期:2014-03-23 出版日期:2014-09-15 发布日期:2014-09-01
  • 通讯作者: 王振华 E-mail:wfzcwzh@126.com

Effect of polysaccharide nucleic acid fraction of bacillus Calmette?鄄Guerin on CD4+CD25+Foxp3+ regulatory T cells in peripheral blood of patients with condyloma acuminatum

  • Received:2014-02-26 Revised:2014-03-23 Online:2014-09-15 Published:2014-09-01
  • Contact: zhenhua wang E-mail:wfzcwzh@126.com

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摘要： 目的 探讨卡介菌多糖核酸（BCG-PSN）对尖锐湿疣（CA）患者外周血CD4+CD25+Foxp3+调节性T细胞（Treg细胞）的影响。 方法 42例首次发病的CA患者分成联合治疗组（26例）和电灼组（16例），联合治疗组给予电灼后肌内注射BCG-PSN 0.35 mg隔日1次连续3个月，电灼组只进行电灼治疗，在初诊时和3个月后分别进行Treg细胞的检测，观察疗效及Treg细胞水平的变化。另有健康体检者30例作为健康对照组。 结果 CA患者外周血Treg细胞水平明显高于健康对照组（8.31% ± 1.24% 比 5.15% ± 0.72%，P < 0.01），其中复发组较未复发组高（9.34% ± 0.72%比7.45% ± 0.85%，P < 0.01）；联合治疗组复发率明显低于电灼组（30.77% 比 68.75%，P < 0.05），3个月后Treg细胞水平下降，明显低于电灼组（5.87% ± 1.05%比6.60% ± 0.75%，P < 0.05）。 结论 Treg细胞比值的高低与CA患者疾病的转归有密切的关系，比值越高越容易复发。BCG-PSN可通过降低Treg细胞的数量来调节CA患者的抗病毒免疫应答，改善其预后。

关键词: 尖锐湿疣, 卡介菌多糖核酸, CD4-阳性T淋巴细胞, FoxP3

Abstract: Wang Zhenhua, Yang Fanghong, Sun Yi, Wang Hui, Xue Feng. Department of Dermatology, Weifang People′s Hospital, Weifang 261041, Shandong, China Corresponding author: Wang Zhenhua, Email: wfzcwzh@126.com 【Abstract】 Objective To evaluate the effect of polysaccharide nucleic acid fraction of bacillus Calmette-Guerin （BCG-PSN） on peripheral blood CD4+CD25+Foxp3+ regulatory T （Treg） cells in patients with condyloma acuminatum （CA）. Methods Forty-two patients with first onset of CA were randomly assigned to receive either injection of BCG-PSN （0.35 mg every other day for 3 months） after fulguration （combination group, 26 patients）, or fulguration only （fulguration group, 16 patients）. Venous blood samples were obtained from all the patients at the initial visit and three months after the beginning of treatment, as well as from 30 healthy checkup examinees. The percentage of peripheral Treg cells in CD4+ T lymphocytes was determined by flow cytometry. The recurrence of CA was evaluated during the three months after the beginning of treatment. Results The percentage of peripheral Treg cells in CD4+ T lymphocytes was significantly higher in patients with CA than in the controls （8.31% ± 1.24% vs. 5.15% ± 0.72%, P < 0.01）, and in patients with clinical recurrence of CA than in those without （9.34% ± 0.72% vs. 7.45% ± 0.85%, P < 0.01）. The recurrence rate was significantly lower in the combination group than in the fulguration group（30.77% vs. 68.75%, P < 0.05）. After three months of treatment, the combination group showed lower percentage of Treg cells in CD4+ T cells compared with the fulguration group （5.87% ± 1.05% vs. 6.60% ± 0.75%, P < 0.05）. Conclusions The percentage of Treg cells has a close relationship with the progression of CA, i.e., the higher the percentage, the more frequent the relapse. BCG-PSN may enhance the antiviral immune response in patients with CA and improve their prognosis by reducing the number of Treg cells.

Key words: Condylomata acuminata, BCG-PSN, CD4-positive T-lymphocytes, FoxP3

中图分类号: 

  • R759