中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (6): 397-399.

• 论著 • 上一篇    下一篇

药疹患者人类疱疹病毒7型感染的检测

张洋1,2,3,陈官芝4,朱桂芝5,汤占利4,陈宏泉4,郭小燕6   

  1. 1. 北京大学
    2. 青岛大学附属医院
    3. 首都医科大学附属北京佑安医院
    4. 青岛大学医学院附属医院
    5. 青岛市中心医院
    6. 菏泽市郓城县人民医院
  • 收稿日期:2013-09-30 修回日期:2013-12-04 发布日期:2014-06-01
  • 通讯作者: 陈官芝 E-mail:chenguanzhi@outlook.com

Detection of human herpesvirus type 7 infection in patients with drug eruptions

  • Received:2013-09-30 Revised:2013-12-04 Published:2014-06-01

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摘要： 目的 探讨人类疱疹病毒7型（HHV-7）感染在药疹发病中的作用。 方法 收集50例药疹患者（其中重型药疹15例）及50例健康人外周血，采用PCR检测外周血单一核细胞中HHV-7 DNA特异性片段；酶联免疫吸附试验（ELISA）检测血清HHV-7 IgM水平。 结果 50例药疹患者HHV-7 DNA阳性率（82.00%，41例）高于健康对照组（62.00%，31例），两组差异有统计学意义（χ2 = 4.96，P < 0.05）；重型药疹患者（93.33%，14/15）、轻型药疹患者（77.14%，27/35）及健康对照组HHV-7 DNA阳性率差异有统计学意义（χ2 = 6.32，P < 0.05），其中重型组高于对照组（q = 3.50，P < 0.05）；重型药疹患者急性期与恢复期HHV-7 DNA阳性率差异无统计学意义（P > 0.05）。药疹患者HHV-7 IgM水平（69.3190 ± 25.2897 ng/L）高于健康对照组（59.7853 ± 22.4382 ng/L，t = 1.99，P < 0.05）；轻型药疹（65.4791 ± 21.3261 ng/L）、重型药疹（74.3407 ± 31.4112 ng/L）及健康对照组间差异均无统计学意义（均P > 0.05）。HHV-7 DNA阳性与阴性药疹患者HHV-7特异性IgM水平（分别为63.7481 ± 27.2391 ng/L、65.5802 ± 36.2584 ng/L）差异无统计学意义（P > 0.05）。 结论 药疹患者中存在HHV-7活动性感染，HHV-7感染可能是药疹发病或加重的因素。

关键词: 药疹, 疱疹病毒7型，人

Abstract: Zhang Yang*, Chen Guanzhi, Zhu Guizhi, Tang Zhanli, Chen Hongquan, Guo Xiaoyan. *Department of Dermatology, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266000, China Corresponding author: Chen Guanzhi, Email: chenguanzhi@outlook.com 【Abstract】 Objective To investigate the role of human herpesvirus type 7 （HHV-7） in the development of drug eruptions. Methods Venous blood samples were collected from 35 patients with mild drug eruptions at acute stage, 15 patients with severe drug eruptions at both acute stage and remission stage, as well as 50 healthy human controls. PCR was performed to detect HHV-7 DNA in peripheral blood mononuclear cells （PBMCs）, and enzyme-linked immunosorbent assay （ELISA） to determine the titer of anti-HHV-7 IgM antibody in serum. Statistical analysis was carried out by t test, one way analysis of variance, Chi-square test and q test. Results The detection rate of HHV-7 DNA was significantly higher in these patients with drug eruptions than in the healthy controls （82.00% （41/50） vs. 62.00% （31/50）, χ2 = 4.96, P < 0.05）, different among patients with severe drug eruptions （93.33% （14/15））, patients with mild drug eruptions （77.14% （27/35）） and the healthy controls （χ2 = 6.32, P < 0.05）, higher in the patients with severe drug eruptions than in the healthy controls （q = 3.50, P < 0.05）, but not significantly different between the patients with severe drug eruptions at acute stage and those at remission stage （73.33%（11/15）, P > 0.05）. The anti-HHV-7 IgM antibody titer was significantly increased in the patients with drug eruptions compared with the healthy controls （（69.319 0 ± 25.289 7） ng/L vs. （59.785 3 ± 22.438 2） ng/L, t = 1.99, P < 0.05）, but no significant difference was observed among the patients with severe drug eruptions （74.340 7 ± 31.411 2） ng/L）, patients with mild drug eruptions （（65.479 1 ± 21.326 1） ng/L） and healthy controls （P > 0.05） or between HHV-7 DNA-positive patients （（63.748 1 ± 27.239 1 ） ng/L） and -negative patients （（65.580 2 ± 36.258 4） ng/L, P > 0.05）. Conclusions Active HHV-7 infection exists in patients with drug eruptions, and may be associated with the development and aggravation of this entity.

Key words: Drug eruptions, Herpesvirus 7, human

引用本文

张洋 陈官芝 朱桂芝 汤占利 陈宏泉 郭小燕. 药疹患者人类疱疹病毒7型感染的检测[J]. 中华皮肤科杂志, 2014,47(6):397-399. doi: