中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (4): 275-277.

• 研究报道 • 上一篇    下一篇

神经生长因子及其高亲和受体酪氨酸激酶、低亲和公共受体p75NTR在扁平苔藓皮损中的表达及意义

钱悦1,陈思远2,黄长征3,冯爱平4,褚淑娟5   

  1. 1. 武汉协和医院皮肤科
    2. 武汉市华中科技大学同济医学院附属协和医院皮肤科
    3. 华中科技大学同济医学院附属协和医院皮肤科
    4. 武汉市协和医院皮肤科
    5. 华中科技大学同济医学院协和医院麻醉科
  • 收稿日期:2013-06-24 修回日期:2013-09-05 出版日期:2014-04-15 发布日期:2014-04-01
  • 通讯作者: 褚淑娟 E-mail:chuchu623@sohu.com
  • 基金资助:
    国家自然科学基金

Expressions of nerve growth factor and its high-affinity receptor, tyrosine kinase A, as well as low-affinity common receptor, p75 neurotrophin receptor, in the lesions of lichen planus and their clinical significance

  • Received:2013-06-24 Revised:2013-09-05 Online:2014-04-15 Published:2014-04-01

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摘要： 【摘要】 目的 检测神经生长因子（NGF）及其受体TrkA、p75NTR在扁平苔藓皮损中的表达。 方法 应用免疫组化ABC法检测32例扁平苔藓皮损和12例健康人皮肤石蜡标本NGF及其受体TrkA、p75NTR表达状况。 结果 NGF及TrkA在32例扁平苔藓皮损表皮角质形成细胞中均有不同程度的表达（++ ～ +++），表达部位为细胞质，高于健康人皮肤NGF（- ～ +）及TrkA（- ～ +）的表达，两组间差异均有统计学意义（P < 0.01）；而p75NTR的表达两组差异无统计学意义。扁平苔藓皮损中NGF与TrkA表达呈正相关（R2 = 0.535，P < 0.01）。NGF及其受体TrkA、p75NTR在扁平苔藓不同发病年龄、部位以及不同性别患者角质形成细胞中的表达差异均无统计学意义。 结论 NGF通过其高亲和受体TrkA在扁平苔藓的发病中可能起着一定的作用。

关键词: 扁平苔藓, 神经生长因子, 蛋白酪氨酸激酶类, p75NTR

Abstract: Qian Yue*, Chen Siyuan, Huang Changzheng, Feng Aiping, Chu Shujuan. *Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China Corresponding author: Chu Shujuan, Email: chuchu623@sohu.com 【Abstract】 Objective To detect the expressions of nerve growth factor （NGF） and its receptors tyrosine kinase A （TrkA） as well as p75 neurotrophin receptor （p75NTR） in the lesions of lichen planus. Methods Biopsy specimens were collected from the lesions of 32 patients with lichen planus and normal skin of 12 healthy human controls and subjected to paraffin embedding. Immunohistochemical avidin-biotin complex （ABC） method was used to detect the expressions of NGF, TrkA and p75NTR. Results NGF and TrkA, which were located in the cytoplasm of keratinocytes, were strongly or moderately expressed in the lesional skin specimens, but absent or weakly expressed in the normal skin specimens （both P < 0.01）. No significant differences were observed in the expression of p75NTR between the lesional and normal skin specimens, or in the expressions of NGF, TrkA or p75NTR among specimens from patients in different age groups, patients of different gender or lesions at different sites （all P > 0.05）. There was a positive correlation between the expression of NGF and TrkA in the lesions of lichen planus （R2 = 0.535, P < 0.01）. Conclusion NGF may play a certain role in the development of lichen planus via its high-affinity receptor TrkA.

Key words: Lichen planus, Nerve growth factor, Protein-tyrosine kinases, p75NTR