中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (1): 53-54.

• 研究报道 • 上一篇    下一篇

慢性特发性荨麻疹患者相关转录因子FOXP3、RORγt基因的表达失衡

朱慧兰1,杨婧2,李润祥2,梁碧华2,梁艳华3,毕超3   

  1. 1. 广州市皮肤防治所
    2. 广州市皮肤病防治所
    3.
  • 收稿日期:2013-01-29 修回日期:2013-03-05 发布日期:2014-01-01
  • 通讯作者: 朱慧兰 E-mail:zhlhuilan@126.com
  • 基金资助:
    广东省自然科学基金

Imbalanced expression of transcription factors FOXP3 and RORγt in patients with chronic spontaneous urticaria

  • Received:2013-01-29 Revised:2013-03-05 Published:2014-01-01
  • Contact: huilan zhu E-mail:zhlhuilan@126.com

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摘要： 【摘要】 目的 探讨Treg/Th17细胞在慢性特发性荨麻疹发病机制中的作用。 方法 89例CSU患者分为活动期组48例，静止期组41例，同期我市社区医院健康体检者48例作为对照组。用荧光定量PCR检测检测研究对象外周血单一核细胞（PBMC）中FOXP3、RORγt mRNA的表达水平。 结果 活动期组PBMC FOXP3 mRNA表达水平（0.57 ± 0.19）显著高于静止期组（0.11 ± 0.21）和健康对照组（0.13 ± 0.23），差异有统计学意义（P < 0.05）。活动期组PBMC RORγt mRNA表达水平（0.43 ± 0.39）显著低于健康对照组（0.87 ± 0.43）和静止期组（0.89 ± 0.40），差异有统计学意义（P < 0.05）。 结论 调控Treg细胞的相关转录因子FOXP3表达水平升高，调控Th17细胞的相关转录因子RORγt表达降低，两种基因的相互作用导致Treg/Th17细胞失衡可能是 CSU 发生的原因之一。

关键词: 荨麻疹, T淋巴细胞，调节性, Th17细胞, 转录因子

Abstract: Zhu Huilan*, Yang Jing, Li Runxiang, Liang Bihua, Liang Yanhua, Bi Chao. *Guangzhou Institute of Dermatology, Guangzhou 510095, China Corresponding author: Zhu Huilan, Email: zhlhuilan@hotmail.com 【Abstract】 Objective To investigate the role of regulatory T （Treg） / T helper type 17 （Th17） cells in the pathogenesis of chronic spontaneous urticaria （CSU）. Methods Eighty-nine patients with CSU were enrolled in this study, including 48 in active stage and 41 in remission stage. Forty-eight health check-up examinees, who were collected from the community hospitals in Guangzhou city, served as the healthy controls. Fluorescence-based real-time quantitative PCR was performed to determine the expression of transcription factors FOXP3 and RORγt in PBMCs from these subjects. Results Compared with the patients with CSU in remission stage and healthy controls, the patients in active stage showed a significantly higher level of FOXP3 mRNA （ 0.57 ± 0.19 vs. 0.11 ± 0.21 and 0.13 ± 0.23, both P < 0.05）, but a significantly lower level of RORγt mRNA （0.43 ± 0.39 vs. 0.89 ± 0.40 and 0.87 ± 0.43, both P < 0.05）. Conclusions The expression of Treg cell regulator FOXP3 increases, while the expression of Th17 cell regulator RORγt decreases in patients with CSU, suggesting that the imbalance between Treg and Th17 cells induced by the interaction between FOXP3 and RORγt may be involved in the pathogenesis of CSU.

Key words: Urticaria, T-lymphocytes, regulatory, Th17 cells, Transcription factors

引用本文

朱慧兰 杨婧 李润祥 梁碧华 梁艳华 毕超. 慢性特发性荨麻疹患者相关转录因子FOXP3、RORγt基因的表达失衡[J]. 中华皮肤科杂志, 2014,47(1):53-54. doi: