中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (9): 640-643.

• 论著 • 上一篇    下一篇

银屑病患者皮损Toll样受体4、核因子-κBp65、CC趋化因子配体20的表达

王爱民1,陆捷洁2,许玉军3,乔凤4,陆玉珠4,杨先旭2,张明3,吴伟伟2,刘巧5,吴晓强6,王玲2,陈大强2,张华7   

  1. 1. 海南省皮肤病医院皮肤性病科
    2. 海南省皮肤病医院
    3. 海口，海南省皮肤病医院皮肤科
    4. 海南省皮肤病医院检验科
    5. 江西中医药大学第二附属医院
    6. 海口 海南省皮肤病医院皮肤性病科
    7. 海口 海南省皮肤病医院皮肤科
  • 收稿日期:2012-10-29 修回日期:2012-11-28 出版日期:2013-09-15 发布日期:2013-09-01
  • 通讯作者: 王爱民 E-mail:w650927@163.com
  • 基金资助:
    海南省自然科学基金

Expressions of Toll-like receptor 4, nuclear factor-kappa B p65 and chemokine CCL20 in psoriatic lesions

  • Received:2012-10-29 Revised:2012-11-28 Online:2013-09-15 Published:2013-09-01
  • Supported by:
    Natural Science Foundation of Hainan Province of China

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摘要： 【摘要】 目的 探讨Toll样受体4（TLR4）、核因子（NF）-κBp65、CC趋化因子配体20（CCL20）在银屑病患者皮损区及非皮损区的表达及意义。方法 用免疫组化EnVision法检测40例进行期银屑病患者斑块状皮损及20例进行期银屑病患者非皮损区皮肤、10例正常皮肤石蜡标本中的TLR4、NF-κBp65、CCL20的表达，对其在皮损中的表达水平进行相关性分析。结果 银屑病皮损区角质形成细胞中TLR4、NF-κBp65、CCL20的表达（A值分别为0.3006 ± 0.1200、0.4551 ± 0.1572和0.3862 ± 0.1304）较正常人皮肤TLR4、NF-κBp65、CCL20的表达（A值分别为0.1379 ± 0.0663、0.1970 ± 0.0679和0.1262 ± 0.0612）及银屑病非皮损区组（A值分别为0.1930 ± 0.0687、0.2626 ± 0.0606和0.2387 ± 0.0467）明显上调（t值分别为4.113、5.044、6.106、3.711、5.265和4.889，均P < 0.05）。与正常人皮肤相比，银屑病非皮损区TLR4、NF-κBp65、CCL20表达明显上调（t值分别为2.118、2.685和5.608，均P < 0.05）。银屑病患者TLR4、NF-κBp65、CCL20的表达水平间均呈正相关（r值分别为0.766、0.851和0.885，均P < 0.05）。结论 TLR4、NF-κBp65、CCL20的高表达可能与银屑病发病有关。 【关键词】 银屑病； Toll样受体4； NF-κBp65； CCL20

Abstract: WANG Ai-min, LU Jie-jie, XU Yu-jun, QIAO Feng, LU Yu-zhu, YANG Xian-xu, ZHANG Ming, WU Wei-wei, LIU Qiao, WU Xiao-qiang, WANG Ling, CHEN Da-qiang, ZHANG Hua. Department of Dermatology and Venereology, Hainan Provincial Center for Skin Disease and STD Control, Haikou 570206, China 【Abstract】 Objective To investigate the expressions of Toll-like receptor 4 （TLR4）, nuclear factor-kappa B （NF-κB） p65 and chemokine CCL20 in lesional and nonlesional skin of patients with progressive plaque-type psoriasis, as well as their clinical implications. Methods Paraffin-embedded tissue samples were prepared with lesional （n = 40） and nonlesional （n = 20） skin from progressive plaque-type psoriatic patients as well as from the normal skin of 10 human controls. Then, the expressions of TLR4, NF-κBp65 and CCL20 were detected by immunohistochemical staining technique using the EnVision antibody complex. Differences in the expression of these parameters were assessed by t test or corrected t test, and the correlations between these proteins were evaluated by Pearson′s product-moment correlation coefficient. Results The expression levels of TLR4, NF-κBp65 and CCL20 were significantly higher in lesional skin than in nonlesional skin from patients and in normal skin from normal controls （TLR4: 0.3006 ± 0.1200 vs. 0.1930 ± 0.0687 and 0.1379 ± 0.0663, t = 5.044, 4.113, respectively, both P < 0.05; NF-κBp65: 0.4551 ± 0.1572 vs. 0.2626 ± 0.0606 and 0.1970 ± 0.0679, t = 3.711, 6.106, respectively, both P < 0.05; CCL20: 0.3862 ± 0.1304 vs. 0.2387 ± 0.0467 and 0.1262 ± 0.0612, t = 4.889, 5.265, respectively, both P < 0.05）, and higher in nonlesional skin of patients than in normal skin of the controls （t = 2.118, 2.685, 5.608, respectively, all P < 0.05）. Moreover, the expressions of TLR4, NF-κBp65 and CCL20 were positively correlated with each other in lesional skin of the patients （r = 0.766, 0.851, 0.885, respectively, all P < 0.05）. Conclusion The up-regulated expressions of TLR4, NF-κBp65 and CCL20 might be involved in the pathogenesis of psoriasis. 【Key words】 Psoriasis; Toll-like receptor 4; NF-κBp65; CCL20

Key words: TLR4, NF-kBp65 , CCL20

中图分类号: 

  • R758.63