中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (8): 561-564.

• 论著 • 上一篇    下一篇

原发性乳房外Paget病组织中钠氢交换子调节因子1和β联蛋白的表达和意义

段紫钰1,张韡2,李阿梅3,吕雅琳4,陈浩5,曾学思5,孙建方5   

  1. 1. 河南省人民医院
    2. 中国医学科学院皮肤病研究所
    3. 南京市 中国医学科学院皮肤病研究所
    4. 中国医学科学院北京协和医学院皮肤病研究所
    5. 南京 中国医学科学院北京协和医学院皮肤病研究所
  • 收稿日期:2012-10-16 修回日期:2013-05-10 出版日期:2013-08-15 发布日期:2013-08-01
  • 通讯作者: 曾学思 E-mail:xsizeng@163.com
  • 基金资助:
    2011协和青年基金

Expressions of NHERF1 and β-catenin in primary extramammary Paget′s disease tissue and their significance

  • Received:2012-10-16 Revised:2013-05-10 Online:2013-08-15 Published:2013-08-01

摘要: 【摘要】 目的 探讨钠氢交换子调节因子1(NHERF1)和β联蛋白在原发性乳房外Paget病组织中的表达及其意义。 方法 应用免疫组化方法,检测18例原位乳房外Paget病、22例侵袭性乳房外Paget病石蜡组织切片中NHERF1和β联蛋白的表达情况。 结果 NHERF1在22例侵袭性乳房外Paget病中18例(81.82%)为高表达,18例原位乳房外Paget病中7例(38.89%)高表达,两组差异有统计学意义(χ2 = 7.78,P < 0.01);β联蛋白在侵袭性乳房外Paget病中有0例胞膜高表达和18例(81.82%)胞质或核高表达,原位乳房外Paget病中分别为6例(33.33%)和8例(44.44%),两组差异均有统计学意义(χ2值分别为8.63和6.08,P值分别 < 0.01和 < 0.05)。原发性乳房外Paget病组织中NHERF1的表达与β联蛋白胞膜表达呈负相关(ρ = -0.488,P < 0.01),与β联蛋白胞质或核表达呈正相关(ρ = 0.623,P < 0.01);β联蛋白胞膜表达与胞质或核表达呈负相关(ρ = -0.572,P < 0.01)。 结论 乳房外Paget病组织中NHERF1和β联蛋白表达异常,并可能与原发性乳房外Paget病的发生发展及侵袭有关。 【关键词】 佩吉特病,乳腺外; β连环素; 钠氢交换子调节因子1

关键词: 佩吉特病,乳腺外, β连环素, 钠氢交换子调节因子1

Abstract: DUAN Zi-yu, ZHANG Wei, LI A-mei, LV Ya-lin, CHEN Hao, ZENG Xue-si, SUN Jian-fang. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China Corresponding author: ZENG Xue-si, Email: xsizeng@163.com 【Abstract】 Objective To investigate the expressions of Na+/H+ exchanger regulatory factor 1 (NHERF1) and β-catenin in extramammary Paget′s disease tissue as well as their significance. Methods Immunohistochemistry was performed to detect the protein expressions of NHERF1 and β-catenin in paraffin-embeded tissue samples from 18 patients with in situ and 22 patients with invasive extramammary Paget′s disease. Results There was a high expression of NHERF1 protein in 18 (81.82%) invasive and 7 (38.89%) in situ extramammary Paget′s disease samples (χ2 = 7.78, P < 0.01). Statistical differences were observed in the membrane expression rate and cytoplasmic or nuclear expression rate of β-catenin between invasive and in situ extramammary Paget′s disease tissue samples (0 (0/22) vs. 33.33% (6/18), χ2 = 8.63, P < 0.01; 81.82% (18/22) vs. 44.44% (8/18), χ2 = 6.08, P < 0.05). In extramammary Paget′s disease in situ tissue samples, the expression of NHERF1 was negatively correlated with the cytomembrane expression of β-catenin(ρ = -0.488, P < 0.01), but positively correlated with the cytoplasmic or nuclear expression of β-catenin (ρ = 0.623, P < 0.01), and there was a negative correlation between the cytomembrane and cytoplasmic or nuclear expression of β-catenin (ρ = -0.572, P < 0.01). Conclusions There is an abnormal expression of NHERF1 and β-catenin in extramammary Paget′s disease tissue, which may be associated with the initiation, progression, and invasion of primary extramammary Paget′s disease. 【Key words】 Paget′s disease, extramammary; Beta catenin; NHERF1