中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (6): 404-407.

• 论著 • 上一篇    下一篇

T细胞受体γ引物组合检测蕈样肉芽肿基因重排的研究

陈柳青1,陈金波2,段逸群1,李东升1,董碧麟1,张红梅1,俞鑫1   

  1. 1. 武汉市第一医院皮肤科
    2. 武汉市第一医院
  • 收稿日期:2012-07-09 修回日期:2013-02-20 出版日期:2013-06-15 发布日期:2013-06-01
  • 通讯作者: 陈柳青 E-mail:chlq35@126.com
  • 基金资助:
    武汉市卫生局科研项目

Application of BIOMED-2 primers in the detection of T cell receptor γ gene rearrangements in patients with mycosis fungoides

  • Received:2012-07-09 Revised:2013-02-20 Online:2013-06-15 Published:2013-06-01
  • Contact: Liu-qing CHEN E-mail:chlq35@126.com

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摘要： 【摘要】 目的 探讨BIOMED-2系统T细胞受体（TCR）γ引物组合在蕈样肉芽肿（MF）患者不同来源标本中TCRγ基因重排检测中的价值。方法 收集28例MF患者15份石蜡组织样本、14份新鲜皮损及18份全血组织样本，提取DNA，利用BIOMED-2系统TCRγ引物组合进行TCRγ基因重排检测，比较3组不同来源样本的检出率并进行统计学分析。用SPSS13.0软件进行统计分析。各组之间阳性率采用χ2检验或Fisher确切概率法检验。结果 15份石蜡包埋组织标本3份TCRγ基因重排阳性，18份血液标本11份阳性，14份新鲜皮损标本12份阳性，3组之间阳性率差异有统计学意义（χ2 = 13.047，P < 0.01），新鲜皮损TCRγ基因重排阳性率显著高于石蜡组织。2011年的6例石蜡标本3份基因重排阳性，阳性率显著高于其余9例2011年之前的石蜡标本（Fisher精确概率法，P = 0.044），与14例新鲜组织标本TCRγ基因重排阳性率（12/14）相比差异无统计学意义（Fisher精确概率法，P = 0.131）。血液标本TCRγ基因重排阳性率低于新鲜皮损，两组之间差异无统计学意义（χ2 = 2.358，P > 0.05）。结论 BIOMED-2系统TCRγ引物组合适用于检测MF患者不同组织样本的TCR基因重排，更适用于新鲜的组织标本。 【关键词】 基因重排，γ链T细胞抗原受体； 肉芽肿，蕈样； 聚合酶链反应

关键词: 蕈样肉芽肿, 基因重排,γ链T细胞抗原受体, 聚合酶链反应

Abstract: CHEN Liu-qing, CHEN Jin-bo, DUAN Yi-qun, LI Dong-sheng, DONG Bi-lin, ZHANG Hong-mei, YU Xin. Department of Dermatology, Wuhan First Hospital, Wuhan 430022, China 【Abstract】 Objective To estimate the value of BIOMED-2 primers for the detection of T cell receptor γ （TCR-γ） gene rearrangements in different types of specimens from patients with mycosis fungoides （MF）. Methods Totally, 15 paraffin-embedded tissue specimens, 14 fresh tissue specimens and 18 whole blood specimens were obtained from 28 patients with MF, and subjected to DNA extraction. BIOMED-2 multiplex PCR tubes TCRγ （A+B） were used for the analysis of TCRγ gene rearrangements. Data were processed by SPSS 13.0 software, and statistical analysis was done by chi-square test and Fisher's exact probability test. Results TCRγ gene rearrangements were detected in 3 paraffin-embedded tissue specimens, 11 fresh tissue specimens and 12 blood specimens, with significant differences in the detection rate between the three samples （χ2 = 13.047, P < 0.01）. The fresh tissue samples showed a significantly higher detection rate than the paraffin-embedded tissue samples （χ2 = 12.523, P < 0.01）. The detection rate of TCRγ gene rearrangements was 3/6 in paraffin-embedded tissue samples collected in 2011, significantly higher than that in the other 9 paraffin-embedded tissue samples collected before 2011 （Fisher′s exact probability test, P = 0.044）, but similar to that in 14 fresh tissue specimens （12/14, Fisher′s exact probability test, P = 0.044）. Decreased detection rate of TCRγ gene rearrangements was observed in blood samples compared with fresh tissue specimens, but no statistical difference was observed between the two types of specimens （χ2 = 2.358, P > 0.05）. Conclusions BIOMED-2 multiplex PCR tubes TCRγ（A+B） are suitable for the detection of clonal rearrangements of TCRγ gene in different types of specimens, especially in fresh tissue specimens, from patients with MF. 【Key words】 Gene rearrangement, gamma-chain T-cell antigen receptor; Mycosis fungoides; Polymerase chain reaction

Key words: PCR