中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (9): 631-633.

• 论著 • 上一篇    下一篇

生存素、Ki67在皮肤结外鼻型NK/T细胞淋巴瘤中的表达及其意义

王婷婷1,阚蓓2,王琳1,李俸媛3,刘卫平4,李甘地5   

  1. 1. 成都市四川大学华西医院皮肤科
    2. 成都市第二人民医院皮肤科
    3. 成都四川大学华西医院病理科
    4. 成都华西医科大学第一附属医院皮肤科
    5. 成都市四川大学华西医院病理科
  • 收稿日期:2011-09-29 修回日期:2011-11-07 出版日期:2012-09-15 发布日期:2012-08-31
  • 通讯作者: 王琳 E-mail:lkzwl@126.com

Expressions of survivin and Ki67 and their significance in cutaneous extranodal NK/T-cell lymphoma, nasal type

  • Received:2011-09-29 Revised:2011-11-07 Online:2012-09-15 Published:2012-08-31

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摘要：

目的 探讨生存素、Ki67在皮肤结外鼻型NK/T细胞淋巴瘤中的表达及意义。方法 选取确诊的以皮损为首发表现的15例皮肤结外鼻型NK/T细胞淋巴瘤为研究对象，常规SP免疫组化染色法检测生存素、Ki67。每张切片选取5个有代表性的高倍镜视野，每个视野随机计数200个肿瘤细胞，分别计算1000个肿瘤细胞内生存素、Ki67阳性细胞百分比。结果 15例皮肤结外鼻型NK/T细胞淋巴瘤中，9例瘤细胞表达CD56，13例表达CD3ε，15例表达TIA-1，10例表达粒酶B，2例表达CD3。15例均表达1 ～ 2个T细胞抗原（CD2、CD45RO或CD7），1例表达βF1，3例表达CD30的病例阳性细胞均为大瘤细胞。所有病例均不表达CD4、CD5、CD8、CD20和CD79α。14例中3例被检出TCR-γ基因克隆性重排，15例EBER1/2原位杂交均阳性。15例中，11例（73.3%）表达生存素，阳性细胞表达率为23.97% ± 18.35%；14例（15例中1例掉片）均表达Ki67，阳性细胞表达率41.20% ± 19.52%。核分裂0 ～ 2个/高倍视野的9例Ki67表达阳性细胞率为25.27% ± 12.96%，核分裂 ＞ 2个/高倍视野的6例为58.23% ± 16.02%，两组差异有统计学意义（F = 19.14，P = 0.001）。皮肤结外鼻型NK/T细胞淋巴瘤中生存素、Ki67都有较高的表达，生存素与Ki67表达之间无相关性。结论 生存素、Ki67的高表达可能在皮肤结外鼻型NK/T细胞淋巴瘤发生发展中起着一定作用。

关键词: 皮肤 淋巴瘤 NK/T细胞 Survivin Ki67

Abstract:

Objective To investigate the expressions of survivin and Ki67 in cutaneous extranodal NK/T-cell lymphoma, nasal type and their significance. Methods Clinical data and laboratory test results were collected from 15 cases of cutaneous extranodal NK/T-cell lymphoma, nasal type with skin lesions as the initial manifestation. Immunohistochemical SP method was used to measure the expression of survivin and Ki67 in tissue sections from the lesions of these patients. To determine the percentage of survivin- or Ki67-positive cells, 200 tumor cells were counted in a high power field （HPF） and 5 HPFs were observed in 1 section. Results There were 10 males and 5 females among the 15 patients, with the median age at diagnosis being 28.7 （range: 9-62） years. Immunohistochemical study showed that the lesional tissue was positive for CD56 in 9 cases, CD3ε in 13 cases, T cell intracellular antigen （TIA）-1 in 15 cases, granzyme B in 10 cases, CD3 in 2 cases, βF1 in 1 case, but negative for CD4, CD5, CD8, CD20, and CD79α. All the 15 cases were positive for 1 or 2 T-cell associated antigens （CD2, CD7 or CD45RO）. CD30 was observed in large tumor cells in 3 of the 15 cases. Monoclonal TCR-γ gene rearrangement was found in 3 of 14 cases. In situ hybridization for EBER1/2 was positive in all of the 15 cases. Survivin and Ki67 were expressed in 73.3% （11/15） and 100% （14/14, note: a tissue section was unavailable because of the detachment of tissue from the slide） of the specimens respectively, with the average percentage of positive cells being 23.97% ± 18.35% and 41.20% ± 19.52% for survivin and Ki67, respectively. Statistical differences were observed in the percentage of Ki67-expressing cells between the specimens with 0-2 mitotic figures per high power field from 9 cases and those with more than 2 mitotic figures from 6 cases （25.27% ± 12.96% vs. 58.23% ± 16.02%, F = 19.14, P = 0.001）. No correlation was found between the expression of survivin and Ki67 in cutaneous extranodal NK/T-cell lymphoma, nasal type. Conclusion The high expressions of survivin and Ki67 may play a role in the occurrence and development of cutaneous extranodal NK/T-cell lymphoma, nasal type.

Key words: Cutaneous lymphoma, NK/T cell, Survivin, Ki67