中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (7): 513-514.

• 研究报道 • 上一篇    下一篇

自制防晒乳膏体外及豚鼠在体防晒性能的评价

马志超,林银花,张晶,周欣,宋洪涛   

  1. 南京军区福州总医院药学科
  • 收稿日期:2011-12-20 修回日期:2012-02-05 出版日期:2012-07-15 发布日期:2012-07-02
  • 通讯作者: 宋洪涛 E-mail:sohoto@vip.sohu.com
  • 基金资助:

    南京军区医学科学技术研究“十一五”计划重点课题

Photoprotective effect of a self-made sunscreen cream: an evaluation in vitro and in vivo by using guinea pigs

  • Received:2011-12-20 Revised:2012-02-05 Online:2012-07-15 Published:2012-07-02

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摘要：

目的 观察自制防晒乳膏的防晒指数SPF值和PFA值。 方法 体外法测定样品防晒指数：将样品以2 μl/cm2的剂量涂抹于仿生皮肤3M膜上，然后用防晒指数测定仪测定样品的防晒指数。豚鼠在体防晒指数的测定：白化豚鼠48只，随机分为8组，其中4组不涂防晒样品，4组分别涂布自制防晒乳膏和对照样品（某市售防晒制剂），使用SUV-1000型日光紫外线模拟器模拟紫外线照射，测定样品防晒指数。结果 体外法检测自制防晒乳膏和某市售制剂的SPF值分别为32.26 ± 2.42和30.87 ± 2.57，PFA值分别是24.28 ± 2.44和17.53 ± 2.28，两种制剂SPF相比，t = 0.94，P ＞ 0.05（n = 5）；但自制防晒乳膏对UVA的防护效果优于某市售制剂，t = 4.52，P ＜ 0.01（n = 5）。豚鼠在体法检测自制防晒乳膏和某市售制剂的SPF值分别为30.39 ± 6.65和28.79 ± 7.36，PFA ＞ 8.91和 ＞ 8.93，两种制剂SPF值比较，t = 0.38，P ＞ 0.05（n = 12）。结论 自制防晒乳膏和某市售制剂相比，对紫外线的防护效果相当。

关键词: 豚鼠在体法

Abstract:

Objective To determine the sun protection factor （SPF） and protection factor of ultraviolet A （PFA） of a self-made sunscreen cream. Methods The sample of a self-made sunscreen cream and a commercial sunscreen cream were painted to an artificial skin （3M film） at a dose of 2 μl/cm2, and a tester was used to evaluate their photoprotective effect. A total of 48 albinism guinea pigs were classified into 8 groups to remain unprotected or be protected by the self-made or commercial sunscreen cream. A solar ultraviolet light simulator （SUV-1000） was used to simulate the ultraviolet rays in sunlight to irradiate the guinea pigs, and the photoprotective effect of these sunscreen creams was determined. Results The in vitro evaluation revealed that the SPF value of the self-made sun-screen cream and commercial cream was 32.26 ± 2.42 and 30.87 ± 2.57 respectively （n = 5, t = 0.94, P > 0.05）, and the PFA value was 24.28 ± 2.44 and 17.53 ± 2.28 respectively （n = 5, t = 4.52, P < 0.01）. As the in vivo experiment showed, the SPF value of the self-made sun-screen cream and commercial cream was 30.39 ± 6.65 and 28.79 ± 7.36, respectively （n = 12, t = 0.38, P > 0.05） , and the PFA value was > 8.91 and > 8.93 respectively. Conclusion The photoprotective effect of the self-made sunscreen cream is similar to that of the commercial cream.

Key words: In-vivo of guinea pigs

中图分类号: 

  • R94