中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (7): 478-480.

• 论著 • 上一篇    下一篇

银屑病患者皮损和外周血中CD123+BDCA-2+ 浆细胞样树突细胞的表达

陈静1,王豫平2,李家文3,陈宏翔3,李丽娜4,李振鲁1   

  1. 1. 郑州市河南省人民医院皮肤科
    2. 河南省人民医院
    3. 华中科技大学同济医学院附属协和医院
    4. 河南省人民医院皮肤性病科；毕业于四川大学华西医院皮肤性病科
  • 收稿日期:2011-09-30 修回日期:2011-11-10 出版日期:2012-07-15 发布日期:2012-07-02
  • 通讯作者: 李振鲁 E-mail:lizhenlu@sohu.com

Quantification of CD123+ BDCA?鄄2+ plasmacytoid dendritic cells in skin lesions and peripheral blood of patients with psoriasis vulgaris

  • Received:2011-09-30 Revised:2011-11-10 Online:2012-07-15 Published:2012-07-02

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摘要：

目的 探讨CD123+BDCA-2+浆细胞样树突细胞（PDC）在寻常性银屑病患者皮损和外周血中的表达及意义。方法 收集22例寻常性银屑病患者和15例健康人皮肤组织和外周血，免疫组化法检测患者皮损和健康人皮肤组织中PDC的表达水平，流式细胞仪检测外周血中PDC的比例。结果 免疫组化结果显示，银屑病皮损中PDC的表达水平为（10.1 ± 2.1）/mm2，健康对照组为（0.4 ± 0.6） /mm2，银屑病组显著高于健康对照组（t = 17.34，P < 0.01）。银屑病患者外周血中CD123+BDCA-2+PDC占外周血单一核细胞的比例为（0.17 ± 0.07）%，健康对照组为（0.33 ± 0.20）%，银屑病组显著低于健康对照组（t = 4.48，P < 0.01）。结论 PDC通过在皮损中重新分布，在银屑病外周血中下降，可能与银屑病的发生、发展相关。

关键词: 树突状细胞

Abstract:

Objective To quantify CD123+ BDCA-2+ plasmacytoid dendritic cells （PDCs） in skin lesions and peripheral blood of patients with psoriasis vulgaris, and to investigate their significance. Methods Skin tissue samples were resected from the lesions of 22 patients with psoriasis vulgaris and normal skin of 15 healthy controls, and peripheral blood samples were also obtained from these subjects. Immunohistochemical technique （SP） was used to quantify PDCs in skin tissue samples, and flow cytometry to determine the proportion of PDCs in peripheral blood samples. Results Immunohistochemical study showed that the density of PDCs in the psoriatic lesions was significantly higher than that in the healthy controls （（10.1 ± 2.1）/mm2 vs.（0.4 ± 0.6）/mm2, t = 17.34, P < 0.01）. On the contrast, the proportion of PDCs in peripheral blood was 0.17％ ± 0.07％ in the patients with psoriasis vulgaris, significantly lower than that in the healthy controls（0.33％ ± 0.20％, t = 4.48, P < 0.01）. Conclusions The proportion of PDCs is reduced in the peripheral blood of patients with psoriasis vulgaris, which may be related to the initiation and progression of psoriasis vulgaris.

Key words: Dendritic Cell