中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (5): 337-339.

• 论著 • 上一篇    下一篇

热处理、UVB辐射及两者联合作用对人表皮黑素细胞合成热休克蛋白72的影响

邵丽芳1,赵广2,牛建荣3,孟如松2,宋文婷1,张悦4,成玉5,闫文厅1   

  1. 1. 解放军空军总医院
    2. 中国人民解放军空军总医院皮肤科 100142
    3. 空军总医院皮肤科
    4. 空军总医院
    5.
  • 收稿日期:2011-08-26 修回日期:2011-10-28 出版日期:2012-05-15 发布日期:2012-05-03
  • 通讯作者: 赵广 E-mail:guangfen@yahoo.com

Effects of heat treatment and UVB radiation alone or in combination on the expression of heat shock protein 72 in human epidermal melanocytes

  • Received:2011-08-26 Revised:2011-10-28 Online:2012-05-15 Published:2012-05-03

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摘要：

目的 探讨热处理、中波紫外线（UVB）辐射及两者联合作用下，体外培养的人表皮黑素细胞热休克蛋白72（heat shock protein 72，HSP72）的变化。 方法 热处理（42 ℃，1 h/d，连续3 d）、UVB辐射（50 mJ/cm2，连续3 d）及两者联合（首先42 ℃，加热1 h，然后 50 mJ/cm2 UVB照射，连续3 d）处理正常人表皮（包皮）黑素细胞2 h、6 h后收集细胞，用实时荧光定量PCR技术和 Western 印迹技术分别检测不同组别间HSP72 mRNA及蛋白表达水平的差异。结果 热处理组（6.584 ± 0.871）及联合作用组（7.269 ± 0.454）与对照组（0.975 ± 0.089）相比，mRNA 表达水平明显增加（P < 0.001）；UVB辐射组（0.832 ± 0.084）与对照组相比，mRNA表达水平差异无统计学意义（P > 0.05）。Western 印迹结果显示，热处理组（2.022 ± 0.058）及联合作用组（2.080 ± 0.045）HSP72蛋白表达水平明显高于对照组（0.532 ± 0.033，P < 0.001）；UVB组（0.546 ± 0.021）与对照组相比，表达水平差异无统计学意义（P > 0.05）。析因设计方差分析结果显示，热处理与UVB辐射对HSP72 mRNA和蛋白表达均无交互作用（F = 2.106，1.399，P < 0.05）。结论 热处理引起HSP72表达显著增多，可能与热处理引起黑素细胞功能增强及UVB辐射后的损伤保护作用有关。

关键词: 热休克蛋白72

Abstract:

Objective To explore the effects of heat treatment and ultraviolet B （UVB） radiation alone or in combination on the expression of heat shock protein （HSP） 72 in human epidermal melanocytes. Methods Melanocytes were obtained from human foreskin, and subjected to primary culture. After 3 to 5 passages, the melanocytes were classified into 4 groups: control group （receiving no treatment）, heat treatment group （treated with heat at 42 ℃ for 1 hour every day for 3 days）, UVB group （irradiated with UVB at 50 mJ/cm2 daily for 3 days）, combination group （treated with heat at 42 ℃ for 1 hour followed by irradiation with UVB at 50 mJ/cm2 daily for 3 days）. After another 2- to 6-hour culture following the last treatment, melanocytes were collected and subjected to real time PCR and Western blot for the detection of HSP72 mRNA and protein expression, respectively. Results The mRNA and protein expressions of HSP72 were significantly higher in the heat treatment group and combination group than in the control group （mRNA: 6.584 ± 0.871 and 7.269 ± 0.454 vs. 0.975 ± 0.089, both P < 0.001; protein: 2.022 ± 0.058 and 2.080 ± 0.045 vs. 0.532 ± 0.033，both P < 0.001）, but was similar between the UVB group and control group （mRNA: 0.832 ± 0.084 vs. 0.975 ± 0.089, P > 0.05; protein: 0.546 ± 0.021 vs. 0.532 ± 0.033, P > 0.05）. The ANOVA of factorial design showed that neither heat treatment nor UVB irradiation had interaction effect on the mRNA or protein expression of HSP72（F = 2.106, 1.399 respectively, both P < 0.05）. Conclusions Heat treatment can cause an increase in the expression of HSP72, which may enhance the function of melanocytes and protect melanocytes from UVB induced damage.

Key words: Heat shock protein 72

中图分类号: 

  • R75