中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (5): 315-317.

• 论著 • 上一篇    下一篇

临床标本中沙眼衣原体噬菌体Vp1基因及血清Vp1抗体的检测

李玲杰1,刘原君2,姚卫锋3,侯淑萍1,田敬群1,冯斌3,刘全忠3   

  1. 1. 天津医科大学总医院皮肤科
    2. 天津医科大学总医院/天津性传播疾病研究所
    3. 天津医科大学总医院皮肤性病科
  • 收稿日期:2011-07-18 修回日期:2011-08-20 出版日期:2012-05-15 发布日期:2012-05-03
  • 通讯作者: 刘全忠 E-mail:liuquanzhong@medmail.com.cn
  • 基金资助:

    国家自然科学基金项目

Detection of Chlamydia trachomatis phage Vp1 gene in clinical swab specimens as well as anti?鄄Vp1 antibodies in serum specimens

  • Received:2011-07-18 Revised:2011-08-20 Online:2012-05-15 Published:2012-05-03
  • Contact: quanzhong liu E-mail:liuquanzhong@medmail.com.cn

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摘要：

目的 检测临床标本中沙眼衣原体噬菌体Vp1基因及血清Vp1抗体。 方法 收集天津性传播疾病研究所就诊患者的分泌物拭子和血清；用PCR法筛查分泌物标本中的Vp1基因；以Vp1蛋白作为抗原通过ELISA法及Western 印迹法检测血清中针对Vp1抗体的存在；对PCR法结果阳性的分泌物进行细胞培养，然后免疫荧光法进行检测Vp1。 结果 共筛查出36例拭子的PCR扩增产物在目的片段位置出现明显条带，及23例Vp1抗体阳性的血清。通过细胞培养及免疫荧光法，利用制备的单抗检测沙眼衣原体临床标本，尚未发现阳性标本。结论 从临床拭子及血清中成功筛查出沙眼衣原体噬菌体Vp1基因和Vp1抗体。

关键词: 沙眼衣原体

Abstract:

Objective To detect Chlamydia trachomatis phage Vp1 gene in clinical swab specimens and anti-Vp1 antibodies in serum specimens. Methods Cervical and urethral swab as well as serum specimens were collected from attendees to the sexually transmitted disease （STD） clinic in the Tianjin Institute of STD, during March 2008 to March 2011. PCR was conducted to detect chlamydial phage Vp1 gene in swab samples, enzyme linked immunosorbent assay （ELISA） and Western blot to detect anti-Vp1 antibody in sera. The swab specimens positive for Vp1 gene were subjected to cell culture followed by the detection of Vp1 protein with an immunofluorescence-based method. Results Totally, 36 out of 1542 swab specimens turned out to be positive for Vp1 gene, and 23 out of 453 serum specimens for anti-Vp1 antibody. No positive results were obtained in the Vp1 gene-positive swab specimens by cell culture and immunofluorescence-based assay. Conclusion The Vp1 gene of Chlamydial trachomatis phage and anti-Vp1 antibody are successfully detected from clinical swab and serum specimens respectively.

Key words: chlamydia trachomatis