中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (4): 279-281.

• 研究报道 • 上一篇    下一篇

过敏性紫癜患者外周血CD146的表达及意义

李玉娟1,王秀敏2   

  1. 1. 滨州医学院 临床学院
    2. 山东省滨州医学院附属医院皮肤科
  • 收稿日期:2011-06-15 修回日期:2011-12-09 出版日期:2012-04-15 发布日期:2012-03-30
  • 通讯作者: 王秀敏 E-mail:wangxiumin@medmail.com.cn

Expression and significance of CD146 in peripheral blood of patients with Henoch-Schonlein purpura

  • Received:2011-06-15 Revised:2011-12-09 Online:2012-04-15 Published:2012-03-30

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摘要：

目的 检测CD146在过敏性紫癜（HSP）患者血清及外周血单一核细胞（PBMC）中的表达，探讨其在HSP发病机制中的意义。方法 采用双抗体夹心酶联免疫吸附试验（ELISA）检测HSP患者急性期、恢复期及正常人对照组血清中可溶性CD146（sCD146）水平；采用实时荧光定量RT-PCR法检测PBMC中CD146 mRNA的相对表达水平，并分析CD146与疾病活动的相关性。结果 HSP患者急性期、恢复期及正常人对照组sCD146水平分别为（0.84 ± 0.49）、（0.52 ± 0.15） 和（0.23 ± 0.08） ng/L，急性期高于恢复期（t = 3.44，P < 0.05），恢复期高于正常人对照组（t = 8.90，P < 0.05）。HSP患者急性期、恢复期及正常人对照组PBMC中CD146 mRNA的相对表达水平分别为26.34 ± 2.55、14.18 ± 2.49和10.08 ± 2.08，急性期明显高于恢复期（t = 18.69，P < 0.05），恢复期高于正常人对照组（t = 6.92，P < 0.05）。结论 CD146可能在HSP的发病中起一定作用。

关键词: CD146

Abstract:

Objective To detect the expression of CD146 in sera and peripheral blood mononuclear cells（PBMCs）of patients with Henoch-Schonlein purpura （HSP）, and to assess its role in the pathogenesis of HSP. Methods Sixty patients with HSP in acute or recovery stage and 20 normal human controls were included in this study． Enzyme-linked immunosorbent assay（ELISA）was performed to measure the expression levels of serum soluble CD146 （sCD146）, and real-time fluorescence-based quantitative reverse transcription-PCR to detect the mRNA expression levels of CD146 in PBMCs. The correlation of the above parameters with disease activity was assessed． Results The levels of serum sCD146 and CD146 mRNA in PBMCs in patients with HSP in recovery stage were significantly higher than those in the normal human controls （0.52 ± 0.15） ng/L vs. （0.23 ± 0.08） ng/L, t = 8.90，P < 0.05; 14.18 ± 2.49 vs. 10.08 ± 2.08, t = 6.92，P < 0.05）, but lower than those in patients with HSP in acute stage （0.84 ± 0.49） ng/L, t = 3.44，P < 0.05; 26.34 ± 2.55, t = 18.69，P < 0.05）. Conclusion CD146 may participate in the pathogenesis of HSP.

Key words: CD146