中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (3): 173-177.

• 论著 • 上一篇    下一篇

非标记定量技术研究瘢痕疙瘩的蛋白质组学特点

杨立群1,陈晓栋2,姚晓东3   

  1. 1. 盐城市第三人民医院
    2. 南通大学附属医院皮肤科
    3. 南通大学附属医院
  • 收稿日期:2011-04-06 修回日期:2011-10-30 出版日期:2012-03-15 发布日期:2012-02-29
  • 通讯作者: 陈晓栋 E-mail:dermatochen@163.com
  • 基金资助:

    国家自然科学基金;江苏省“六大人才高峰”第七批项目;南通大学研究生创新工程项目

Application of label-free quantitative proteomics in keloid

  • Received:2011-04-06 Revised:2011-10-30 Online:2012-03-15 Published:2012-02-29
  • Contact: Chen Xiao-Dong E-mail:dermatochen@163.com

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摘要：

目的 联合应用毛细管高效液相色谱与串联质谱技术，寻找瘢痕疙瘩组织表达的差异蛋白，探索其蛋白质组学特点。方法 选取非家族性瘢痕疙瘩患者病变组织为实验组，以正常皮肤组织为对照，分别提取两组蛋白质，经溶液内酶解获得肽段，应用液相色谱串联质谱鉴定和DeCyder MSTM软件进行非标记定量和蛋白质差异统计分析。结果 对比分析瘢痕疙瘩和正常皮肤组织，共鉴定到570个有显著性差异的肽段（P < 0.05），对应1221个差异表达的蛋白质，其中差异表达超过1.5倍的蛋白质293个，124个在瘢痕疙瘩组织中表达上调，169个表达下调。结论 非家族性瘢痕疙瘩患者病变组织与正常皮肤组织之间存在蛋白质表达差异。

关键词: 非家族型

Abstract:

Objective To identify differentially expressed proteins in keloid tissue and to explore the proteomic characteristics of keloid by utilizing capillary high-performance liquid chromatography （LC） and tandem mass spectrometry （MS）. Methods Tissue specimens were obtained from the lesions of 5 patients with non-familial keloid and normal skin of 5 human controls. After extraction and in-solution digestion of proteins, the resultant peptides were separated and identified by LC-MS/MS. Label-free detection and quantitation of peptides were carried out by using the DeCyder MSTM software. Results A total of 570 differentially expressed peptides were identified between non-familial keloid and normal tissue （all P < 0.05）, which correspond to 1221 proteins. A difference greater than 1.5 folds was observed in the expressions of 293 proteins, including 124 up-regulated and 169 down-regulated proteins in non-familial keloid tissue. Conclusion Differences exist in the expression of proteins between non-familial keloid and normal skin tissue.

Key words: Non-familial

中图分类号: 

  • R751