中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (2): 81-82.

• 论著 • 上一篇    下一篇

可变脉宽 Nd:YAG激光与强脉冲光治疗鲜红斑痣动物模型的研究

朱雅琳1,李建华2,聂晶1,惠艳3   

  1. 1. 新疆医科大学第一附属医院
    2. 新疆医科大学附属第一附属医院皮肤科
    3. 乌鲁木齐市新疆医科大学第一附属医院
  • 收稿日期:2011-04-07 修回日期:2011-08-28 出版日期:2012-02-15 发布日期:2012-01-31
  • 通讯作者: 惠艳 E-mail:HuiYan168@163.net

Variable pulsed Nd:YAG laser and intense pulsed light for the treatment of port wine stain in animal models

  • Received:2011-04-07 Revised:2011-08-28 Online:2012-02-15 Published:2012-01-31

摘要:

目的 通过动物模型研究可调脉宽Nd:YAG激光与强脉冲光对鲜红斑痣的治疗作用。方法 以38只莱亨鸡作为鲜红斑痣动物模型,随机分为4组,其中1组(2只)作为空白对照组不给予激光照射,其余3组(每组12只)分别给予可变脉宽Nd:YAG激光照射2次、强脉冲光照射2次、Nd:YAG激光和强脉冲光照射各1次,分别观察鸡冠形态学、组织学变化,对照射前后每高倍镜下血管数的变化进行统计学分析。结果 2次照射后,各组鸡冠被照射区颜色变淡,光镜下可见真皮血管层变薄,血管闭锁,血管数目减少。每高倍镜( × 400)下血管数Nd:YAG照射组为(17.92 ± 3.63)个,强脉冲光照射组为(8.08 ± 1.56)个,联合照射组为(7.08 ± 1.31)个,与未照射的对照组(37.08 ± 3.97)个相比,各组血管数均减少,差异均有统计学意义(P < 0.01)。联合照射组与强脉冲光照射组比较,差异无统计学意义(P > 0.01),但低于Nd:YAG组(P < 0.01)。结论 可变脉宽Nd:YAG激光照射、强脉冲光照射、两者联合照射均可用于鲜红斑痣的治疗。强脉冲光照射、二者联合照射治疗鲜红斑痣效果可能优于单独可变脉宽Nd:YAG激光照射。

关键词: 强脉冲光

Abstract:

Objective To study the therapeutic effect of variable pulsed Nd:YAG laser and intense pulsed light (IPL) on port wine stain(PWS) in an animal model. Methods The combs of 38 Leghorn chickens served as the animal model of PWS, which were randomly classified into 4 groups: blank control group receiving no irradiation, Nd:YAG group receiving Nd:YAG laser irradiation for 2 sessions, IPL group receiving IPL irradiation for 2 sessions, and combination group receiving 1 session of Nd:YAG laser irradiation followed by 1 session of IPL irradiation 45 days later. One week after the final irradiation, the morphology of combs was observed, and tissue specimens were resected from the combs for the analysis of histological changes and blood vessel density. The data were assessed by using one-way ANOVA. Results After 2 sessions of irradiation, the color of comb skin was lightened, and light microscopy showed the thickening of vascular layers in the dermis, blockage of blood vessels and decrease in the number of blood vessels. The count of blood vessels per high power field (× 400) was significantly lower in the Nd:YAG group (17.92 ± 3.63), IPL group (8.08 ± 1.56) and combination group (7.08 ± 1.31) than in the control group (37.08 ± 3.97, all P < 0.01), but was similar between the combination group and IPL group (P > 0.01), and higher in the Nd:YAG group than in the combination group (P < 0.01). Conclusions Variable pulsed Nd:YAG laser and IPL, alone or in combination, can be used in the treatment of PWS, however, the effect of IPL alone or in combination with Nd:YAG seems superior to that of Nd:YAG laser alone.

Key words: IPL