中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (2): 138-139.

• 研究报道 • 上一篇    下一篇

犬小孢子菌膜蛋白PQ-LRP基因全长cDNA的克隆

庞娟1,祝逸平2,杨国玲3   

  1. 1. 大连医科大学附属一院皮肤科
    2.
    3. 大连医科大学附属第一医院
  • 收稿日期:2011-04-21 修回日期:2011-05-30 出版日期:2012-02-15 发布日期:2012-01-31
  • 通讯作者: 杨国玲 E-mail:Yanggl@medmail.com.cn
  • 基金资助:

    国家自然科学基金

Cloning of full-length cDNA of Microsporum canis membrane protein PQ-loop repeat protein gene

Juan PANG 2,yang guoling   

  • Received:2011-04-21 Revised:2011-05-30 Online:2012-02-15 Published:2012-01-31
  • Contact: yang guoling E-mail:Yanggl@medmail.com.cn

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摘要：

目的 克隆犬小孢子菌膜蛋白PQ-LRP（PQ-loop repeat protein）基因全长cDNA，探讨在头癣发病机制中的作用。方法 选用犬小孢子菌头癣株（A518）为实验株，采用cDNA快速末端扩增法（RACE），克隆PQ-LRP基因的全长序列。结合生物信息学方法对获得的序列进行初步功能分析。结果 获得犬小孢子菌PQ-LRP全长序列为1522 bp，拥有一个1080 bp 的开放阅读框，编码359个氨基酸，5′非编码区为49 bp，3′非编码区为393 bp；同源性比对与断发毛癣菌的PQ-LRP同源性达到81%，与红色毛癣菌PQ-LRP同源性达到79%。结论 克隆出犬小孢子菌膜蛋白PQ-LRP cDNA 全长序列，为研究膜蛋白PQ-LRP基因在犬小孢子菌病中的功能奠定基础。

关键词: cDNA快速末端扩增法

Abstract:

Objective To clone the full-length cDNA of Microsporum canis membrane protein PQ-loop repeat protein （PQ-LRP） gene, so as to investigate the roles of PQ-LRP in the pathogenesis of tinea capitis. Methods A Microsporum canis strain （A518） from a patient with tinea capitis served as the experimental strain. Rapid cDNA end amplification （RACE） was performed to clone the full length cDNA sequence of PQ-LRP gene. Bioinformatics methods were used to make a preliminary functional analysis of the gene. Results The cDNA of PQ-LRP gene was obtained with a full length of 1522 bp, including the 5′ untranslated region （49 bp）, coding region （1080 bp） and 3′ untranslated region （393 bp）. The coding region encoded a protein precursor including 359 amino acid residues. The cloned cDNA of PQ-LRP gene shared an 81% nucleotide identity with that of Trichophyton tonsurans and a 79% nucleotide identity with that of Trichophyton rubrum. Conclusions The full-length cDNA of Microsporum canis membrane protein PQ-LRP gene has been successfully cloned, which will provide an important basis for further researches into the roles of PQ-LRP in Microsporum canis-associated diseases.

Key words: SMART-RACE

中图分类号: 

  • R379