中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (2): 136-137.

• 研究报道 • 上一篇    下一篇

基质金属蛋白酶2和金属蛋白酶组织抑制因子2在乙酰肝素酶ASODN转染的裸鼠Ａ375细胞移植瘤中的表达

陈建立1,张江安2,于建斌3,孟静4,李琳5,陈燕辉6   

  1. 1. 黄河中心医院
    2. 郑州大学第一附属医院
    3. 郑州大学第一附属医院皮肤科
    4. 郑州大学第一附属医院； 商丘市第一人民医院
    5. 郑州市儿童医院
    6. 郑州人民医院
  • 收稿日期:2011-04-25 修回日期:2011-06-18 出版日期:2012-02-15 发布日期:2012-01-31
  • 通讯作者: 于建斌 E-mail:yjbdoctor@sina.com

Expressions of matrix metalloproteinase-2 and tissue inhibitor of matrix metalloproteinase-2 in human malignant melanoma transplanted in nude mice

  • Received:2011-04-25 Revised:2011-06-18 Online:2012-02-15 Published:2012-01-31

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摘要：

目的 探讨基质金属蛋白酶２（MMP2）和金属蛋白酶组织抑制因子２（TIMP2）在乙酰肝素酶反义寡核苷酸（ASODN）转染的裸鼠恶性黑素瘤移植瘤中的表达。方法 分空白组、无关序列（N-ODN）组、10 μmol/L ASODN组、20 μmol/L ASODN组和30 μmol/L ASODN组5组，以脂质体包埋分别转染人恶性黑素瘤细胞株A375，后接种于裸鼠皮下，待成瘤后采用免疫组化SP法检测移植瘤中MMP2和TIMP2蛋白表达的变化。结果　各ASODN组裸鼠移植瘤中MMP2和TIMP2蛋白表达水平均较对照组、N-ODN组显著下调（P < 0.05）；ASODN3个浓度组（10、20、30 μmol/L）相比，差异均有统计学意义（P < 0.05）。结论　乙酰肝素酶ASODN 对裸鼠体内恶性黑素瘤移植瘤中的MMP2和TIMP2蛋白的表达有显著抑制效应。

关键词: 移植

Abstract:

Objective To investigate the expressions of matrix metalloproteinase-2 （MMP2） and tissue inhibitor of MMP2 （TIMP2） in human malignant melanoma transplanted subcutaneously in nude mice. Methods A non-sense oligonucleotide （N-ODN） and an antisense oligodeoxynucleotide（ASODN）against heparanase mRNA were designed and synthesized. Cultured human A375 malignant melanoma cells were classified into 4 groups to be transfected with the N-ODN, ASODN of 10, 20, 30 μmol/L, respectively, via liposomes. The cells receiving no treatment served as the blank control group. Then, the transfected or untransfected cells were subcutaneously inoculated into nude mice. Six weeks after the transplantation, transplanted tumors were removed from the nude mice and subjected to immunohistochemical staining for the detection of MMP2 and TIMP2 protein expressions. Results The protein expressions of MMP2 and TIMP2 were significantly lower in tumor tissue specimens from nude mice inoculated with ASODN-transfected A375 cells than in those with N-ODN-transfected cells and in those with untransfected cells （P < 0.05）, significantly different between the tumor tissue specimens from mice inoculated with A375 cells transfected with 10, 20 and 30 μmol/L of ASODN （all P < 0.05）. Conclusion The ASODN against heparinase displays a marked inhibitory effect on the expressions of MMP2 and TIMP2 proteins in malignant melanoma transplanted in nude mice.

Key words: Transplantation

中图分类号: 

  • R751