中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (2): 134-135.

• 研究报道 • 上一篇    下一篇

银屑病患者皮肤屏障功能受损的研究

顾华1,李娜2,涂颖2,庞勤3,何黎4   

  1. 1. 昆明医学院第一附属医院皮肤性病科
    2. 昆明医学院第一附属医院皮肤科
    3. 昆明医学院第一附属医院
    4. 昆明市昆明医学院第一附属医院皮肤科
  • 收稿日期:2011-04-25 修回日期:2011-07-07 出版日期:2012-02-15 发布日期:2012-01-31
  • 通讯作者: 何黎 E-mail:Heli2662@yahoo.com.cn

Dysfunction of epidermal barrier in psoriasis

  • Received:2011-04-25 Revised:2011-07-07 Online:2012-02-15 Published:2012-01-31

PDF

38

赞

摘要：

目的 探讨银屑病患者皮肤屏障功能受损的实验依据，以指导临床辅助治疗银屑病。方法 60例银屑病患者运用无创性皮肤生理功能测试仪检测皮损角质层含水量、皮脂含量及经表皮水分流失（TEWL）。电镜观察皮损处细胞超微结构，同时运用免疫组化方法检测皮损处酸性神经酰胺酶的表达。结果 与正常皮肤比较，银屑病皮损角质层含水量降低（P < 0.01），TEWL值增加（P < 0.01），皮脂含量差异无统计学意义。电镜下，皮损颗粒层角质形成细胞中板层小体数量较正常对照组减少，分布紊乱，体积大小不等；免疫组化染色显示酸性神经酰胺酶表达明显减少。结论 银屑病皮肤屏障功能明显受损，因此，恢复皮肤屏障功能，加强保湿是银屑病重要的辅助治疗手段之一。

关键词: 电镜

Abstract:

Objective To seek experimental evidence of epidermal barrier dysfunction in psoriasis, and to provide a basis for adjuvant therapy of psoriasis. Methods Physiometric methods were used to determine the value of sebum content, transepidermal water loss （TEWL） and water content of stratum corneum in 60 patients with psoriasis and 48 normal human controls. The ultrastructure of lamellar bodies was observed with transmission electron microscopy, and the expression of acid ceramidase in normal skin and psoriatic lesions was detected by using immunohistochemical techniques. Results Compared with the normal skin, TEWL value was increased （P < 0.01）, but water content of stratum corneum decreased （P < 0.01） in psoriatic lesions, and sebum content was similar between normal skin and psoriatic lesions. As electron microscopy showed, lamellar bodies in keratinocytes were reduced in number with a disorganized arrangement and irregular size in psoriatic lesions. The expression of acid ceramidase also decreased in psoriatic epidermis. Conclusions The function of epidermal barrier in psoriasis is impaired, and to restore epidermal barrier function and enhance hydration may serve as an important adjuvant therapy of psoriasis.

Key words: transmission electron microscopy