中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (11): 818-819.

• 研究报道 • 上一篇    下一篇

系统性红斑狼疮患者外周血T淋巴细胞激活状态及OX40、OX40L mRNA表达

李桂珍1,张珩2,赵俊芳3,陈雪雯4,王蓉1,王玉亮5   

  1. 1. 天津市中医药研究院附属医院
    2. 天津市大港医院
    3. 天津市长征医院
    4.
    5. 天津医科大学一中心临床学院
  • 收稿日期:2011-11-28 修回日期:2012-06-26 出版日期:2012-11-15 发布日期:2012-10-31
  • 通讯作者: 李桂珍 E-mail:janeliguichen@126.com

Activation status of T lymphocytes and expressions of OX40, OX40L mRNA in peripheral blood from patients with systemic lupus erythematosus

  • Received:2011-11-28 Revised:2012-06-26 Online:2012-11-15 Published:2012-10-31

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摘要：

目的 探讨系统性红斑狼疮（SLE）患者外周血T淋巴细胞激活状态及OX40、OX40L mRNA表达水平。方法 采用双色流式细胞仪测定SLE患者和健康人外周血CD38CD3分子表达，采用荧光定量逆转录聚合酶链反应（RT-PCR）测定外周血单一核细胞（PBMC）中OX40、OX40L mRNA表达水平。结果 SLE患者外周血CD38CD3分子表达水平显著高于健康人（t = 3.12，P < 0.05）。活动期SLE患者PBMC OX40、OX40L mRNA水平显著高于非活动期患者和健康人（F = 4.13，3.12，P值均 < 0.01）。OX40 mRNA水平与SLE疾病活动指数呈正相关（r = 0.35，P < 0.05）。结论 T淋巴细胞异常活化及OX40、OX40L mRNA表达水平上调在SLE的发生、发展中可能起一定作用。

关键词: CD38

Abstract:

Objective To evaluate the activation status of T lymphocytes and to measure the expression of OX40 and OX40L mRNA in peripheral blood from patients with systemic lupus erythematosus （SLE）. Methods Peripheral blood was collected from 30 patients with SLE and 15 healthy controls. The expression of CD38 and CD3 on peripheral blood T lymphocytes were quantified by using a two-color fluorescence activated cell sorter. Fluorescence-based quantitative reverse transcription PCR was performed to determine the expression levels of OX40 and OX40L mRNA in peripheral blood mononuclear cells （PBMCs）. Results The coexpression levels of CD38 and CD3 were significantly increased in patients with SLE compared with healthy controls （t = 3.12, P < 0.05）. Increased expression levels of OX40 and OX40L mRNA were observed in patients with active SLE compared with those with inactive SLE and healthy controls （F = 4.13, 3.12, both P < 0.01）. The level of OX40 mRNA was positively correlated with SLE disease activity index （SLEDAI） score in the patients （r = 0.35, P < 0.05）. Conclusion Abnormally activated T lymphocytes and upregulated expression of OX40 and OX40L mRNA may play a certain role in the initiation and progression of SLE.

Key words: CD38