中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (10): 745-746.

• 研究报道 • 上一篇    下一篇

他克莫司对人Jurkat淋巴瘤细胞分泌IL-6和sIL-2R及表达IL-6和sIL-2R mRNA的影响

张峻岭1,闫翠彦2,索丹凤3,马秀亮4   

  1. 1. 天津市长征医院
    2. 北京市丰台区妇幼保健院皮肤科
    3. 天津市第一中心医院皮肤科
    4. 天津市长征医院皮肤色素科
  • 收稿日期:2011-10-26 修回日期:2012-05-20 出版日期:2012-10-15 发布日期:2012-09-29
  • 通讯作者: 张峻岭 E-mail:zhangjunling2005@yahoo.com.cn
  • 基金资助:

    中华医学会皮肤性病学分会－安斯泰来皮肤病学研究基金

Effects of tacrolimus on the secretion of interleukin-6 （IL-6）, soluble interleukin-2 receptor （sIL-2） and expression of IL-6 and sIL-2R mRNA by a human lymphoma cell line Jurkat

Zhang Jun-Ling 2, 2, 2   

  • Received:2011-10-26 Revised:2012-05-20 Online:2012-10-15 Published:2012-09-29
  • Contact: Zhang Jun-Ling E-mail:zhangjunling2005@yahoo.com.cn

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摘要：

目的 探讨他克莫司对淋巴细胞分泌IL-6和sIL-2R及表达IL-6和sIL-2RmRNA的影响。 方法 酶联免疫吸附试验测定不同浓度他克莫司对人Jurkat淋巴瘤细胞产生IL-6和sIL-2R的影响，实时荧光定量PCR法分析他克莫司对淋巴瘤细胞IL-6 mRNA和sIL-2R mRNA表达的影响。结果 102 ～ 104 nmol/L他克莫司可以抑制Jurkat淋巴瘤细胞分泌IL-6、sIL-2R （P值均 < 0．05），其中103 ～ 104 nmol/L他克莫司作用显著。102 nmol/L他克莫司可下调淋巴瘤细胞IL-6和sIL-2R mRNA的表达 （P值均 < 0．05）。 结论 适当浓度他克莫司可抑制淋巴细胞分泌IL-6和sIL-2R，下调IL-6和sIL-2R mRNA的表达。

关键词: sIL-2RmRNA

Abstract:

Objective To evaluate the effects of tacrolimus on the secretion of IL-6 and sIL-2R as well as the expression of IL-6 and sIL-2R mRNA by lymphocytes. Methods Jurkat human lymphoma cells were cultured and treated with tacrolimus of different concentrations. Enzyme linked immunosorbent assay was performed to determine the levels of IL-6 and sIL-2R in the supernatant of Jurkat cells at 48 hours after treatment with tacrolimus of 0, 10, 102, 103 and 104 nmol/L, and real time reverse transcription PCR to measure the expression of IL-6 mRNA and sIL-2R mRNA of Jurkat cells at 48 hours after treatment with tacrolimus of 102 nmol/L. Results Tacrolimus of 102 － 104 nmol/L could suppress the secretion of IL-6 and sIL-2R from Jurkat cells （all P < 0.05）, with a more marked suppressing effect achieved by the use of tacrolimus at 103 － 104 nmol/L. The expressions of IL-6 and sIL-2R mRNA from Jurkat cells were downregulated by tacrolimus of 102 nmol/L （both P < 0.05）. Conclusion Tacrolimus at certain concentrations could downregulate the secretion of IL-6 and sIL-2R as well as the expression of IL-6 and sIL-2R mRNA by lymphocytes.

Key words: sIL-2RmRNA