中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (10): 714-717.

• 论著 • 上一篇    下一篇

人黑素瘤细胞株IGFBP7基因启动子区CpG岛异常甲基化的研究

薛燕宁1,陈浩2,周武庆3,4,曹玉萍5,陶玥6,曾学思2,孙建方2   

  1. 1. 江苏省中医院皮肤科
    2. 南京 中国医学科学院北京协和医学院皮肤病研究所
    3. 中国医学科学院北京协和医学院皮肤病研究所
    4.
    5. 中国医学科学院皮肤病研究所
    6. 南京大学医学院附属鼓楼医院皮肤性病科
  • 收稿日期:2012-04-01 修回日期:2012-05-02 出版日期:2012-10-15 发布日期:2012-09-29
  • 通讯作者: 孙建方 E-mail:fangmin5758@yahoo.com.cn
  • 基金资助:

    国家自然科学基金

Methylation status of CpG islands in the promoter region of IGFBP7 gene in human melanoma cell lines

  • Received:2012-04-01 Revised:2012-05-02 Online:2012-10-15 Published:2012-09-29
  • Supported by:

    National nature science foundation of China

PDF

42

赞

摘要：

目的 探讨人黑素瘤细胞株和原代黑素细胞IGFBP7启动子区CpG岛甲基化状态与IGFBP7表达的关系。 方法 亚硫酸氢钠修饰后测序法检测4株人黑素瘤细胞和原代黑素细胞中IGFBP7基因启动子区CpG岛DNA甲基化状态。 结果 IGFBP7表达阴性的3个细胞株（A375、M14、SK-MEL-1）和表达阳性的2个细胞株（MV3及原代黑素细胞）的甲基化模式存在差异，聚类分析各自聚为一类。结论 IGFBP7基因启动子区CpG岛甲基化状态和基因表达有一定相关性。

关键词: DNA甲基化

Abstract:

Objective To assess the relationship of methylation status of CpG islands in the promoter region of insulin-like growth factor binding protein 7 （IGFBP7） gene with the expression of IGFBP7 gene in human melanoma cell lines and primary melanocytes. Methods Primary melanocytes from human foreskin tissue as well as 4 human melanoma cell lines, including A375, M14, SK-MEL-1 and MV3, were used in this study. Bisulfite sequencing PCR （BSP） was applied to detect the methylation status of 54 CpG sites in the 5′-flanking promoter region of IGFBP7 gene in all of the melanoma cell lines and primary melanocytes. Results As hierarchical cluster analysis showed, IGFBP7-positive cells （including A375, M14 and SK-MEL-1） differed significantly from IGFBP7-negative cells （including MV3 cells and primary melanocytes） in the methylation pattern of IGFBP7 gene promoter region. Conclusion The methylation status of CpG island in the promoter region of IGFBP7 gene may be associated with its expression in melanoma cell lines.

Key words: DNA methylation