中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (1): 49-52.

• 研究报道 • 上一篇    下一篇

埃兹蛋白、上皮型钙黏着蛋白、桩蛋白、整合素β1蛋白在皮肤鳞状细胞癌中的表达

闫越颖1,张士发1,赵丽萍2,刘静1,刘超1,李淑琴1   

  1. 1. 沈阳军区总医院皮肤科
    2.
  • 收稿日期:2011-02-09 修回日期:2011-08-29 出版日期:2012-01-15 发布日期:2011-12-31
  • 通讯作者: 张士发 E-mail:zhangshifa_1963@126.com

Expressions of ezrin, E-cadherin, paxillin and integrin β1 in skin squamous cell carcinoma

  • Received:2011-02-09 Revised:2011-08-29 Online:2012-01-15 Published:2011-12-31

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摘要：

目的 探讨埃兹蛋白（ezrin）、上皮型钙黏着蛋白（E-cadherin）、桩蛋白（paxillin）和整合素β1（INTβ1）在皮肤鳞状细胞癌（SCC）中的表达及意义。方法 应用免疫组化法对30例SCC患者及10例正常对照者的ezrin、E-cadherin、paxillin和INTβ1表达进行检测。 结果 正常人表皮角质形成细胞上均表达ezrin和E-cadherin（10/10），表达率显著高于SCC肿瘤细胞［56.67%（17/30）和13.33%（4/30），χ2分别为6.42和24.76，P值均 < 0.05］；正常人表皮角质形成细胞上paxillin和INTβ1的表达率为0和10.00%（1/10），均显著低于SCC肿瘤细胞［70.00%（21/30）和66.67%（20/30），χ2分别为14.74和9.66，P值分别 < 0.01和0.05］。SCC肿瘤细胞ezrin和E-cadherin的表达呈显著正相关（r = 0.52，P < 0.01），paxillin和E-cadherin、INTβ1与E-cadherin的表达均呈显著负相关（r值分别为－0.56和－0.52，P值均 < 0.01）；ezrin与paxillin、Ezrin与INTβ1、paxillin与INTβ1的表达无相关性（r值分别为0.5、0.24和0，P值均 > 0.05）。结论 E-cadherin低表达、paxillin和INTβ1高表达可能共同促进肿瘤细胞的侵袭和转移；INTβ1与E-cadherin在SCC转移中可能起到拮抗作用。

关键词: 鳞状细胞癌

Abstract:

Objective To investigate the expressions of ezrin, E-cadherin, paxillin and integrin β1 in skin squamous cell carcinoma （SCC） and their significance. Methods The expressions of ezrin, E-cadherin, paxillin and integrin β1 were immunohistochemically detected in tissue specimens from 30 patients with SCC and 10 normal human controls. Results The expression rates of ezrin and E-cadherin in normal human keratinocytes were significantly higher than those in SCC cells ［100.00% （10/10）vs. 56.67%（17/30）, χ2 = 6.42, P < 0.05; 100.00% （10/10） vs 13.33% （4/30）, χ2=24.76, P < 0.05］, while the expression rates of paxillin and integrin β1 in normal human keratinocytes were significantly lower than those in SCC cells ［0.00 vs. 70.00% （21/30）, χ2 = 14.74, P < 0.01; 10.00% （1/10） vs. 66.67% （20/30）, χ2 = 9.66, P < 0.05］. In SCC cells, there was a significant positive correlation between the expression of ezrin and E-cadherin（r = 0.52, P < 0.01）, but a negative correlation between the expression of paxillin and E-cadherin and between that of integrin β1 and E-cadherin （r = － 0.56, － 0.52 respectively, both P < 0.01）; no significant correlation was observed between the expression of ezrin and paxillin or integrin β1, or between the that of paxillin and integrin β1（r = 0.5, 0.24 and 0 respectively, all P > 0.05）. Conclusions The low expression of E-cadherin and high expression of paxillin and integrin β1 may synergistically promote the invasion and metastasis of tumor cells, while integrin β1 and E-cadherin may play an antagonistic role in the metastasis of SCC.

Key words: squamous cell carcinoma