中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (1): 29-31.

• 论著 • 上一篇    下一篇

血管内皮细胞和角质形成细胞对血卟啉单甲醚吸收特性的比较

李若虹1,周展超2,林彤2,孙建方2   

  1. 1. 卫生部中日友好医院
    2. 南京 中国医学科学院北京协和医学院皮肤病研究所
  • 收稿日期:2011-02-18 修回日期:2011-10-18 出版日期:2012-01-15 发布日期:2011-12-31
  • 通讯作者: 周展超 E-mail:Zhanchao_zhou@yahoo.com.cn

Comparison of absorption characteristics of hematoporphyrin monomethyl ether （HMME） by ECV304 cells versus HaCaT cells

1,zhan-chao ZHOU 1,   

  • Received:2011-02-18 Revised:2011-10-18 Online:2012-01-15 Published:2011-12-31
  • Contact: zhan-chao ZHOU E-mail:Zhanchao_zhou@yahoo.com.cn

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摘要：

目的 探讨血管内皮细胞（ECV304）和角质形成细胞（HaCaT）对光敏剂血卟啉单甲醚（hematoporphyrin monomethyl ether, HMME）的吸收特性。方法 取对数生长期的ECV304和HaCaT细胞分别与50、100、150、200、250 mg/L HMME孵育16 h，或与150 mg/L HMME孵育15 min、30 min、1 h、3 h、8 h、12 h、24 h。激光扫描共聚焦显微镜检测孵育浓度及孵育时间对两种细胞吸收HMME的影响。结果 与上述5种浓度HMME孵育后，ECV304细胞的平均荧光强度分别为74.00、125.57、135.24、141.99、132.09，HaCaT细胞的平均荧光强度分别为93.88、102.45、112.59、108.23、104.70。与150 mg/L HMME孵育上述时间后，ECV304细胞的平均荧光强度分别为95.07、103.97、105.96、108.99、112.93、115.36、122.91，HaCaT细胞的平均荧光强度分别为104.25、106.60、108.72、113.75、117.66、114.90、118.14。结论 ECV304和HaCaT细胞对HMME的吸收在一定浓度范围和时间范围内均呈浓度和时间依赖性。

关键词: 光动力治疗

Abstract:

Objective To compare the absorption characteristics of HMME by a human umbilical vein endothelial cell line ECV 304 versus a human keratinocyte cell line HaCaT. Methods Exponentially growing ECV304 and HaCaT cells were incubated with various concentrations （50, 100, 150, 200 and 250 mg/L） of HMME for 16 h or HMME of 150 mg/L for various durations （15 min, 30 min, 1 h, 3 h, 8 h, 12 h and 24 h）. The quantity of HMME absorbed by the cells were determined by laser scanning confocal microscopy （LSCM）. Results The fluorescence intensity was 74.00, 125.57, 135.24, 141.99 and 132.09 for ECV304 cells, 93.88, 102.45, 112.59, 108.23 and 104.70 for HaCaT cells, after incubation with HMME of 50, 100, 150, 200 and 250 mg/L, respectively. After treated with HMME of 150 mg/L for 15 min, 30 min, 1 h, 3 h, 8 h, 12 h and 24 h, ECV304 cells showed a fluorescence intensity of 95.07, 103.97, 105.96, 108.99, 112.93, 115.36 and 122.91, respectively, and HaCaT cells displayed a fluorescence intensity of 104.25, 106.60, 108.72, 113.75, 117.66, 114.90 and 118.14, respectively. Conculsions Within a defined range of concentration and duration, the absorption of HMME by both ECV304 and HaCaT cells is, to some extent, concentration- and time-dependent.

Key words: Photodynamic therapy