中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (9): 633-635.

• 论著 • 上一篇    下一篇

HLA-DQA1*0302、DQB1*0303与新疆维吾尔族白癜风的相关性研究

辛宁1,唐小辉2,3,陈军2,4,康晓静5   

  1. 1. 山东省即墨市人民医院病理科
    2.
    3. 新疆维吾尔自治区人民医院
    4. 石河子大学医学院 2008级研究生
    5. 乌鲁木齐市 新疆维吾尔自治区人民医院皮肤科
  • 收稿日期:2010-12-06 修回日期:2011-01-13 出版日期:2011-09-15 发布日期:2011-08-31
  • 通讯作者: 康晓静 E-mail:drkangxj666@yahoo.com.cn
  • 基金资助:

    新疆维吾尔自治区科技攻关和重点科技项目

Association of HLA-DQA1*0302, DQB1*0303 alleles with vitiligo in Uygur nationality in Xinjiang Uygur Autonomous Region

  • Received:2010-12-06 Revised:2011-01-13 Online:2011-09-15 Published:2011-08-31

PDF

44

赞

摘要：

目的 探讨HLA-DQA1、DQB1等位基因与新疆维吾尔族白癜风相关性。方法 聚合酶链反应-序列特异性引物（PCR-SSP）检测300例维吾尔族白癜风患者HLA-DQA1*0302、DQB1*0303等位基因。结果 与300例维吾尔族正常人对照组相比，①白癜风患者DQA1*0302（20.5%比13.83%）、DQB1*0303（30.17%比13.33%）等位基因频率显著增高（P ＜ 0.01）；②HLA-DQA1*0302、DQB1*0303等位基因频率在成人型（发病年龄 ＞ 12岁）及儿童型（发病年龄≤12岁）的白癜风患者中均增高（P ＜ 0.01）；③HLA-DQB1*0303等位基因频率在有、无家族史的白癜风患者中均增高（P ＜ 0.01），HLA -DQA1*0302等位基因频率在无家族史病例中显著增高（P ＜ 0.01）；④白癜风组儿童型和成人型两组间比较及有、无家族史两组间比较，DQA1*0302、DQB1*0303等位基因频率差异无统计学意义（P > 0.05）。 结论 HLA-DQA1*0302、DQB1*0303等位基因可能与新疆维吾尔族白癜风相关，儿童型和成人型及有、无家族史的白癜风在其遗传背景上可能存在异质性。

关键词: 聚合酶链反应-序列特异性引物技术

Abstract:

Objective To identify the association of HLA-DQA1*0302 and DQB1*0303 alleles with vitiligo in Uygur nationality in Xinjiang Uygur Autonomous Region. Methods Polymerase chain reaction with sequence-specific primers （PCR-SSP） was performed to analyze the distribution of HLA-DQA1*0302 and HLA-DQB1*0303 alleles among 300 patients with vitiligo and 300 normal human controls of Uygur nationality in Xinjiang region. Results A significant increase was observed in the frequency of HLA-DQA1*0302 and -DQB1*0303 alleles in patients with vitiligo compared with the controls （20.5% vs. 13.83%, 30.17% vs. 13.33%, both P < 0.01）. Increased frequency of HLA-DQA1*0302 and -DQB1*0303 alleles was also seen in patients with adult vitiligo （onset age > 12 years） and those with childhood vitiligo （onset age≤12 years） as compared with the normal controls （both P < 0.01）. The frequency of DQB1*0303 allele was higher in both patients with and without family history of vitiligo than in the normal controls （both P < 0.01）, while that of DQA1*0302 was higher in only patients without family history （P < 0.01）. No significant difference was observed in the frequency of HLA-DQA1*0302 or HLA-DQB1*0303 between patients with adult vitiligo and those with childhood vitiligo or between patients with and without family history （all P > 0.05）. Conclusions HLA-DQA1*0302 and DQB1*0303 alleles may be associated with vitiligo in Uygur nationality in Xinjiang region, and there seems to be genetic heterogeneity between patients with adult and childhood vitiligo and between vitiligo patients with and without family history.

Key words: PCR-SSP

中图分类号: 

  • R758.4+1