中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (7): 491-493.

• 论著 • 上一篇    下一篇

玻璃酸钠对黑素细胞生物学活性的影响

洪为松1,钱国培1,许爱娥2   

  1. 1. 杭州市第三人民医院
    2. 安徽医科大学附属杭州市第三人民医院皮肤科
  • 收稿日期:2010-03-01 修回日期:2011-03-13 出版日期:2011-07-15 发布日期:2011-07-12
  • 通讯作者: 许爱娥 E-mail:xuaiehz@msn.com
  • 基金资助:

    浙江省科技厅重大科技专项国际合作项目;杭州市科技局

Effects of hyaluronic acid on biological activity of melanocytes

  • Received:2010-03-01 Revised:2011-03-13 Online:2011-07-15 Published:2011-07-12

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摘要：

目的 观察玻璃酸钠对黑素细胞增殖及酪氨酸酶活性的影响。方法 噻唑蓝法和酪氨酸酶活性测定法分别检测不同浓度玻璃酸钠对正常人黑素细胞生长和黑素合成能力的影响。结果 玻璃酸钠质量浓度为0、0.008、0.016、0.313、0.625、1.250、2.500、5.000、7.500、10.000 g/L时，黑素细胞增殖活性（A490）分别为0.14 ± 0.02、0.37 ± 0.08、0.45 ± 0.11、0.49 ± 0.07、0.55 ± 0.12、0.52 ± 0.11、0.49 ± 0.07、0.39 ± 0.05、0.19 ± 0.03、0.01 ± 0.01，玻璃酸钠质量浓度在0. 08 ~ 5 g/L范围内时，对黑素细胞的增殖有明显促进作用，达10 g/L时会抑制黑素细胞的增长。玻璃酸钠质量浓度在0.2 ~ 5 g/L时可明显促进酪氨酸酶活性，到10 g/L时酪氨酸酶活性反而下降。在接种黑素细胞同时加入玻璃酸钠的黑素细胞增殖作用明显快于接种后8 h再加入玻璃酸钠组。结论 玻璃酸钠在浓度0.2 ~ 5 g/L时既能促进黑素细胞的增殖，又能明显提高黑素细胞的酪氨酸酶活性。

关键词: 移植

Abstract:

Objective To observe the effect of hyaluronic acid on the proliferation of and tyrosinase activity in melanocytes. Methods Normal primary human melanocytes were isolated from infant foreskin tissue and cultured. Different concentrations （0 to 10 g/L） of hyaluronic acid were added to the culture medium immediately or 8 hours after the inoculation of melanocytes. MTT assay was performed to detect the proliferation of melanocytes, and tyrosinase activity was determined to evaluate the effect of hyaluronic acid on the melanin synthesis by melanocytes. Results The proliferation level （absorbance at 490 nm, A490） of melanocytes was 0.14 ± 0.02, 0.37 ± 0.08, 0.45 ± 0.11, 0.49 ± 0.07, 0.55 ± 0.12, 0.52 ± 0.11, 0.49 ± 0.07, 0.39 ± 0.05, 0.19 ± 0.03 and 0.01 ± 0.01 when treated with hyaluronic acid of 0, 0.008, 0.016, 0.313, 0.625, 1.250, 2.500, 5.000, 7.500 and 10.000 g/L, respectively. The hyaluronic acid of 0.08 to 5 g/L markedly accelerated the proliferation of melanocytes, while that of 10 g/L inhibited their proliferation. The tyrosinase activity in melanocytes was promoted by hyaluronic acid of 0.2 to 5 g/L, but suppressed by that of 10 g/L. The proliferation of melanocytes treated with hyaluronic acid immediately after the inoculation was more rapid than that treated with hyaluronic acid 8 hours after the inoculation. Conclusion The hyaluronic acid of 0.2 to 5 g/L can enhance the proliferation of and tyrosinase activity in melanocytes.

Key words: transplantation