寻常性银屑病患者外周血Th17/Treg失衡的研究

中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (6): 411-414.

寻常性银屑病患者外周血Th17/Treg失衡的研究

何荣国¹,伍绍国²,田华*³,武钦学³,邬运学⁴

1. 广州市第十二人民医院皮肤科
2.
3. 广州市第十二医院皮肤科
4. 广州市第十二人民医院

收稿日期:2010-08-18 修回日期:2010-12-11 出版日期:2011-06-15 发布日期:2011-06-02
通讯作者: 何荣国 E-mail:hrg0901@qq.com

Th17/Treg imbalance in the peripheral blood of patients with psoriasis vulgaris

Received:2010-08-18 Revised:2010-12-11 Online:2011-06-15 Published:2011-06-02
Contact: He Rong-Guo E-mail:hrg0901@qq.com

摘要/Abstract

摘要：

目的探讨寻常性银屑病患者外周血辅助性T细胞17（Th17）/调节性T细胞（Treg）的失衡及意义。方法收集48例寻常性银屑病患者外周血，同时以PASI评分评价银屑病的严重程度，流式细胞仪检测48例寻常性银屑病患者和32例正常人外周血Th17、Treg细胞比例， ELISA方法检测患者和正常人血清中IL-17、IL-10表达水平。结果寻常性银屑病患者外周血Th17细胞比例和血清IL-17水平分别为（2.70 ± 1.43）%和（90.65 ± 29.61） ng/L，较正常人组（0.86 ± 0.25）%和（48.82 ± 5.49） ng/L显著升高，差异有统计学意义（P值均 < 0.01）。Treg细胞比例和血清IL-10水平分别为（3.63 ± 1.14）%和（17.78 ± 4.09） ng/L，较正常人组（7.87 ± 1.26）%和（23.76 ± 3.82） ng/L显著下降，差异有统计学意义（P值均 < 0.01）。Th17/Treg和IL-17/IL-10比值分别为0.95 ± 0.76和5.78 ± 3.19，较正常人组（0.12 ± 0.06）和（2.16 ± 0.68）显著升高，差异有统计学意义（P值均 < 0.01）。银屑病患者PASI评分与Th17细胞比例、血清IL-17水平、Th17/Treg细胞和IL-17/IL-10比值呈正相关性（r值分别为0.97、0.93、0.99、0.97，P值均 < 0.01），而与外周血Treg细胞比例和血清IL-10水平均呈负相关性（r值分别为-0.87、-0.90，P值均 < 0.01）。结论Th17/Treg失衡可能在寻常性银屑病的发病机制中具有一定作用。

关键词: 白细胞介素l0

Abstract:

Objective To investigate the imbalance between T helper 17 （Th17） cells and CD4+CD25+ regulatory T （Treg） cells in the peripheral blood of patients with psoriasis vulgaris and its significance. Methods Peripheral blood were collected from 48 patients with psoriasis vulgaris and 32 normal human controls. Pasoriasis area and severity index （PASI） was used to assess the disease severity in these patients. Flow cytometry was performed to determine the percentage of Th17 and Treg cells in peripheral blood, enzyme linked immunosorbent assay （ELISA） to measure the levels of serum interleukin （IL） -17 and IL-10. Results There was a significant increase in the percentage of Th17 cells ［（2.70 ± 1.43）% vs. （0.86 ± 0.25）%, P < 0.01］ and serum IL-17 level （90.65 ± 29.61 ng/L vs. 48.82 ± 5.49 ng/L, P < 0.01）, but a decrease in the percentage of Treg cells ［（3.63 ± 1.14）% vs. （7.87 ± 1.26）%, P < 0.01］ and serum IL-10 level （17.78 ± 4.09 ng/L vs. 23.76 ± 3.82 ng/L, P < 0.01） in patients with psoriasis vulgaris compared with the normal controls. The ratios of Th17 to Treg cells and serum IL-17 to IL-10 level were significantly higher （0.95 ± 0.76 vs. 0.12 ± 0.06, 5.78 ± 3.19 vs. 2.16 ± 0.68, both P < 0.01） in the patients than in the normal controls. The PASI score in patients was positively correlated with the percentage of Th17, serum level of IL-17, Th17/Treg ratio and IL-17/IL-10 ratio （r = 0.97, 0.93, 0.99 and 0.97, all P < 0.01）, but negatively correlated with the percentage of Treg cells and serum IL-10 level（r = -0.87, -0.90, both P < 0.01）. Conclusion The imbalance between Th17 and Treg cells may play an important role in the pathogenesis of psoriasis vulgaris.

Key words: Intedeukin-10

何荣国伍绍国田华* 武钦学邬运学. 寻常性银屑病患者外周血Th17/Treg失衡的研究[J]. 中华皮肤科杂志, 2011, 44(6): 411-414.

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