中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (6): 387-389.

• 论著 • 上一篇    下一篇

新疆头癣紫色毛癣菌分离株的PCR-RFLP基因分型

张巧巧1,帕丽达·阿布利孜1,邓淑文2,3   

  1. 1. 新疆医科大学第一附属医院
    2. 新疆医科大学第一附属医院皮肤科
    3.
  • 收稿日期:2010-10-09 修回日期:2011-01-13 出版日期:2011-06-15 发布日期:2011-06-02
  • 通讯作者: 帕丽达·阿布利孜 E-mail:palidaa@yahoo.com.cn
  • 基金资助:

    国家自然科学基金项目;新疆少数民族科技人才特殊培养计划科研项目

Genotyping of Trichophyton violaceum isolated from children with tinea capitis in Xinjiang Uygur Autonomous Region by PCR-RFLP analysis

  • Received:2010-10-09 Revised:2011-01-13 Online:2011-06-15 Published:2011-06-02
  • Contact: Palida Abulizi E-mail:palidaa@yahoo.com.cn

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摘要：

目的 探讨新疆地区紫色毛癣菌的基因特点，为新疆儿童头癣致病菌的分子流行病学研究提供依据。 方法 用5种限制性内切酶HaeIII，Bgl I，MspI，DdeI 及MboI，采用PCR限制性片段长度多态性（RFLP）法对分离于新疆儿童头癣的30株紫色毛癣菌的rDNA非转录间隔（NTS）区进行基因分型，并与北京的9株、台湾的2株紫色毛癣菌做比较。结果 内切酶DdeI将全部41株菌分为12个基因型。新疆的30株菌分为10个基因型，其中17株菌分为D、F、G、H、I、J、K 7个基因型，与北京和台湾菌株间有明显基因差异，其余13株菌分3个基因型，与北京和台湾菌株有基因同源性。结论 新疆地区儿童头癣分离的紫色毛癣菌既有独特基因型，又与北京、台湾菌株有基因同源性，体现了新疆紫色毛癣菌的基因多态性。

关键词: 基因分型

Abstract:

Objective To analyze the genotypic characteristics of T. violaceum, and offer evidence for molecular epidemiological study of causative agents of pediatric tinea capitis, in Xinjiang Uygur Autonomous Region. Methods The PCR-restriction fragment length polymorphisim （RFLP） of the nontranscribed spacer region of ribosomal DNA （rDNA-NTS） was assessed by using 5 restriction enzymes, including Hae III, Bgl I, MspI，DdeI and MboI, for 30 clinical isolates of T. violaceum from children with tinea capitis in Xinjiang Uygur Autonomous Region. Nine strains of T. violaceum from Beijing and 2 strains from Taiwan served as the control. Results All the 41 strains of T. violaceum were classified into 12 genotypes with the restriction enzyme DdeI. Ten genotypes were revealed in the 30 strains from Xinjiang; among them, 17 strains showed 7 different genotypes （D, F, G, H, I, J and K） with a high intraspecies diversity in comparison with the Beijing and Taiwan isolates; the remaining 13 strains from Xinjiang showed 3 genotypes, which were shared by the Beijing and Taiwan isolates. Conclusions The T. violaceum isolates from children with tinea capitis have unique genotypes, but also show genetic homogeneity with the strains from Beijing and Taiwan, hinting the genetic diversity of T. violaceum.

Key words: genotypes