中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (5): 360-361.

• 研究报道 • 上一篇    下一篇

复发性生殖器疱疹患者血清可溶性PD-1的表达和免疫学意义

吴健1,贾国泉2,吴瑞斌3,胥萍1,陈永井1,吴敏智1,曹莉1,张学光4,张逸成1   

  1. 1.
    2. 苏州市第五人民医院
    3. 江苏省苏州市吴中区皮肤病防治所
    4. 苏州大学医学生物技术研究所
  • 收稿日期:2010-04-21 修回日期:2011-02-09 出版日期:2011-05-15 发布日期:2011-05-03
  • 通讯作者: 贾国泉 E-mail:jiaguoquan@163.com

Expression of soluble PD-1 in the sera of patients with recurrent genital herpes and its immunologic significance

  • Received:2010-04-21 Revised:2011-02-09 Online:2011-05-15 Published:2011-05-03

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摘要：

目的 检测复发性生殖器疱疹（RGH）患者血清中可溶性PD-1（sPD-1）蛋白的浓度，探讨sPD-1的异常表达在HSV-Ⅱ病毒感染中的意义。方法 选择74例RGH患者，其中发作期RGH患者34例，稳定期RGH患者40例，同时选择88例健康献血者作为对照组；应用单克隆抗体标记、ELISA法检测sPD-1的表达情况。结果 RGH组血清sPD-1蛋白水平为（33.06 ± 17.5） μg/L，低于对照组sPD-1水平（53.07 ± 26.3） μg/L，两者比较差异有统计学意义（t = 5.78，P < 0.01）。发作期RGH组血清sPD-1蛋白水平为（27.47 ± 12.9） μg/L，稳定期RGH组为（37.71 ± 19.6） μg/L，两组间差异有统计学意义（P < 0.01）。结论 RGH组血清中sPD-1异常低表达，可能是HSV-Ⅱ逃避机体免疫监视、产生免疫耐受的机制之一。

关键词: 流式细胞仪

Abstract:

Objective To determine the concentration of soluble PD-1 （sPD-1） in the sera of patients with recurrent genital herpes （RGH）, and to explore the significance of abnormal expression of sPD-1 in RGH. Methods Serum samples were obtained from 88 healthy blood donors, 74 patients with RGH including 34 cases of outbreak-stage RGH and 40 cases of stable-stage RGH. The serum level of sPD-1 was measured by monoclonal antibody labeling and enzyme linked immunosorbent assay （ELISA）. Results A significant difference was observed in the serum level of sPD-1 between the patients with RGH and the blood donors （33.06 ± 17.5 μg/L vs. 53.07 ± 26.3 μg/L, P < 0.01） and between the patients with outbreak-stage RGH and those with stable-stage RGH （27.47 ± 12.9 μg/L vs. 37.71 ± 19.6 μg/L, P < 0.01）. Conclusions There is a low expression of sPD-1 in patients with RGH, which may be one of the mechanisms underlying the escape of HSV-Ⅱ from immunologic surveillance and development of immunological tolerance.

Key words: Flow cytometry