中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (5): 352-353.

• 研究报道 • 上一篇    下一篇

淋球菌性菌血症的快速筛查及鉴定

吴意1,聂署萍2,吴正林2,姜海洋2,黄烈2,吴润香3,范菲楠3   

  1. 1. 深圳市第四（福田）人民医院检验医学部
    2. 深圳市第四（福田）人民医院
    3.
  • 收稿日期:2010-08-30 修回日期:2010-10-15 出版日期:2011-05-15 发布日期:2011-05-03
  • 通讯作者: 聂署萍 E-mail:nieping2@126.com
  • 基金资助:

    深圳市福田区卫生系统公益性科研项目

Rapid screening for and confirmation of gonococcemia

  • Received:2010-08-30 Revised:2010-10-15 Online:2011-05-15 Published:2011-05-03

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摘要：

目的 探讨16S rDNA检测技术在快速筛查鉴定淋球菌性菌血症中的应用价值。方法 选择1例反复规律性发热、畏寒1个月入院的41岁男性菌血症患者，入院前几天开始出现尿急、尿频和尿痛症状，大便正常，贫血消瘦外容，皮肤黏膜稍苍白，无出血点及皮疹，全身浅表淋巴结未及肿大，血尿常规异常，肝肾功能尚可。采用PCR检测菌血症患者外周血病原菌16S rDNA，对其扩增产物进行DNA测序，再将DNA序列结果与GenBank中的已知序列进行同源性比较；同时对外周血病原菌进行分离培养鉴定及药敏实验。结果 16S rDNA扩增产物序列与GenBank中的已知序列进行同源性比较后确定为淋病奈瑟菌，同外周血分离培养鉴定结果一致。结论 检测淋球菌性菌血症患者外周血16S rDNA具有高效率、高特异性、高灵敏度、快速准确等特点。

关键词: 菌血症

Abstract:

Objective To investigate the application value of 16S rDNA-based detection technique in the rapid screening for and confirmation of gonococcemia. Methods A 41-year-old male patient was hospitalized for recurrent regular fever and chills for 1 month. Several days before the admission, he developed urgent micturition, frequent micturition and pain in urination, anemia with emaciation appearance, slightly pale-looking skin and mucous membranes. No petechia, skin eruption or superficial lymphadenectasis was observed, but routine blood test and urine test results were abnormal. No abnormality was found in stool test or hepatic and renal function. DNA was extracted from the blood of the patient and subjected to PCR for the amplification of 16S rDNA followed by sequence analysis and homology analysis. At the same time, bacterial culture of blood and drug sensitivity test for the bacterial isolate were performed. Results Homology analysis indicated that the amplicon sequence was consistent with the known sequence of 16S rDNA of Neisseria gonorrhoeae in GeneBank, which agreed well with the culture result of peripheral blood. Conclusion The detection of 16s rDNA with PCR from peripheral blood is highly efficient, specific, sensitive, rapid and accurate for the screening for and confirmation of gonococcemia.

Key words: Bacteremia