白介素22及其相关因子在银屑病患者血清及外周血单一核细胞中的表达

中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (4): 238-240.

白介素22及其相关因子在银屑病患者血清及外周血单一核细胞中的表达

史玉玲¹,徐晓光²,毕新岭³,王英³,卜晓琳⁴,顾军⁵

1. 同济大学附属第十人民医院
2. 第二军医大学附属长海医院皮肤科
3. 上海第二军医大学长海医院皮肤科
4. 第二军医大学附属长海医院
5. 上海第二军医大学附属长海医院皮肤科

收稿日期:2010-08-05 修回日期:2010-09-11 出版日期:2011-04-15 发布日期:2011-04-01
通讯作者: 顾军 E-mail:gujun79@163.com

Expressions of interleukin （IL）-22 and related cytokines in the sera and peripheral blood mononuclear cells （PBMCs） of patients with psoriasis

Received:2010-08-05 Revised:2010-09-11 Online:2011-04-15 Published:2011-04-01
Contact: GU Jun E-mail:gujun79@163.com

摘要/Abstract

摘要：

目的探讨白介素（IL）-22及其相关因子IL-23p19、IL-6在寻常性银屑病患者外周血单一核细胞（PBMC）及血清中表达的意义。方法采用实时定量RT-PCR法检测寻常性银屑病患者及正常人对照者外周血PBMC中IL-22 mRNA、IL-23p19 mRNA及IL-6 mRNA的水平；采用ELISA法检测寻常性银屑病患者及正常人对照者血清和PBMC培养上清中IL-22的分泌水平。结果银屑病组和正常人对照组PBMC中IL-22 mRNA的相对表达量分别为4.48 ± 2.64和2.35 ± 0.91；IL-23p19 mRNA的相对表达量分别为6.07 ± 4.09和2.61 ± 1.46；IL-6 mRNA的相对表达量分别为3.87 ± 1.49和1.48 ± 0.62；差异均有统计学意义（P < 0.01）。ELISA检测发现，银屑病组和正常人对照组血清中IL-22的水平分别为（86.23 ± 25.58） ng/L和（43.67 ± 14.82） ng/L（P < 0.01），PBMC培养上清中IL-22的水平分别为（119.11 ± 21.51） ng/L和（57.70 ± 13.17） ng/L（P < 0.01）。结论寻常性银屑病患者PBMC和血清中过度表达IL-22，提示IL-22可能参与银屑病的发病机制。

关键词: 酶联免疫吸附试验

Abstract:

Objective To investigate the expressions and significance of IL-22 and related cytokines （IL-23p19 and IL-6） in sera and PBMCs of patients with psoriasis. Methods Sera and PBMCs were obtained from the venous blood samples from 58 patients with psoriasis vulgaris and 20 normal human controls. The PBMCs were subjected to culture for 5 hours followed by the collection of cells and culture supernatant. Then, quantitative real-time RT-PCR was used to examine the mRNA expressions of IL-22, IL-23p19 and IL-6 in PBMCs, enzyme-linked immunosorbent assay （ELISA） to detect the level of IL-22 protein in the sera and culture supernatant of PBMCs. Results In the patients with psoriasis and controls, the relative expression level in PBMCs was 4.48 ± 2.64 and 2.35 ± 0.91 respectively for IL-22 mRNA, 6.07 ± 4.09 and 2.61 ± 1.46 respectively for IL-23p19 mRNA, 3.87 ± 1.49 and 1.48 ± 0.62 respectively for IL-6 mRNA; significant differences were observed between the two groups in all the above parameters （all P < 0.01）. ELISA revealed that the level of IL-22 protein in the patients and controls was （86.23 ± 25.58） ng/L and （43.67 ± 14.82） ng/L respectively in the sera （P < 0.01）, （119.11 ± 21.51） ng/L and （57.70 ± 13.17） ng/L respectively in the culture supernatant of PBMCs （P < 0.01）. Conclusion There is an overexpression of IL-22 in the PBMCs and sera of patients with psoriasis, implying that IL-22 is involved in the pathogenesis of psoriasis.

Key words: ELISA

史玉玲徐晓光毕新岭王英卜晓琳顾军. 白介素22及其相关因子在银屑病患者血清及外周血单一核细胞中的表达[J]. 中华皮肤科杂志, 2011, 44(4): 238-240.

参考文献

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