SLE患者雌激素受体-α与相关细胞因子的表达及临床相关性研究

中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (3): 205-208.

SLE患者雌激素受体-α与相关细胞因子的表达及临床相关性研究

陈宏¹,赵会娟¹,常晓萍²,门剑龙³

1. 天津市长征医院皮肤科
2.
3. 天津医科大学总医院临床检验科

收稿日期:2010-07-19 修回日期:2010-11-16 出版日期:2011-03-15 发布日期:2011-03-10
通讯作者: 陈宏 E-mail:baixue20042006@126.com

Expressions of estrogen receptor-α and related cytokines and their clinical correlation in patients with systemic lupus erythematosus （SLE）

Received:2010-07-19 Revised:2010-11-16 Online:2011-03-15 Published:2011-03-10

摘要/Abstract

摘要：

目的探讨SLE患者雌激素受体（ER）对T、B淋巴细胞的作用机制以及T、B淋巴细胞在SLE发病中的协同作用。方法采用实时荧光定量PCR检测40例SLE患者与40例正常人外周血单核细胞（PBMC）ER-α、IL-10、B淋巴细胞刺激因子（BLyS） mRNA表达水平；分析ER-α、IL-10和BLyS mRNA表达与临床及实验室指标的相关性。结果 SLE组ER-α、IL-10、BLyS mRNA相对表达量显著高于正常人对照组（P < 0.05或P < 0.01），SLE活动期较静止期明显增高（P < 0.05或P < 0.01），且其表达水平与肾损害、蛋白尿、关节炎等临床及实验室指标有相关性。男女SLE组以及男女正常对照组的表达比较差异无统计学意义。结论 IL-10和BLyS与SLE的病情活动性和严重程度相关，ER-α可能在SLE T、B淋巴细胞的作用机制中有重要作用。

关键词: 实验室指标

Abstract:

Objective To study the mechanism of effects of estrogen receptor （ER） on T and B lymphocytes in patients with SLE and synergistic effect of T and B lymphocytes in the pathogenesis of SLE. Methods Real-time fluorescence quantitative PCR was performed to detect the mRNA expressions of ER-α, interleukin 10 （IL-10） and B lymphocyte stimulator （BLyS） in peripheral blood mononuclear cells （PBMCs） from 40 SLE patients and 40 normal human controls. The clinical and laboratory correlation with the levels of these parameters was analyzed. Results A significant increase was observed in the relative expression levels of ER-α, IL-10 and BLyS mRNA in SLE patients compared with the normal human controls （P < 0.05 or 0.01）, in active SLE patients compared with inactive SLE patients （P < 0.05 or 0.01）. Additionally, the mRNA expression levels of the 3 parameters were significantly correlated with the presence of renal damage, proteinuria, arthritis, etc. No statistical difference was observed in the mRNA expression levels of these parameters between female and male patients or between female and male normal controls. Conclusions IL-10 and BLyS appear to be correlated with the disease activity and severity of SLE, and ER-α may play an important role in the action mechanism of T and B lymphocytes in SLE.

Key words: experiment index

陈宏赵会娟常晓萍门剑龙. SLE患者雌激素受体-α与相关细胞因子的表达及临床相关性研究[J]. 中华皮肤科杂志, 2011, 44(3): 205-208.

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