应用基因芯片技术筛选皮肤光老化相关基因

摘要/Abstract

摘要：

目的探讨光老化皮肤与正常皮肤的基因表达差异，在转录组水平上探讨光老化分子生物学机制。方法选取2007—2008年入院面部光老化患者6例，收集患者全颜面除皱术中切除的耳前（光曝露）、耳后（光保护）全层皮肤，进行全基因组表达芯片分析，筛选差异基因并进行功能聚类分析。结果 1例因数据差异明显被排除，对其他5例数据分组分析共筛选差异表达基因172个，上调99个，下调73个。筛选出的差异基因涉及细胞黏附及受体调节、信号转导、代谢等多个生物学过程。结论光老化皮肤与正常皮肤之间存在多处基因表达差异。除皱史可能与皮肤光老化基因差异表达程度存在一定联系。

关键词: 基质金属蛋白酶

Abstract:

Objective To detect differentially expressed genes between human normal skin and photoaging skin, and to investigate the molecular and biological mechanisms of human skin photoaging at transcriptional level. Methods Full-thickness skin specimens were obtained during full-face rhytidectomy from sun-exposed （anterior ear skin） and sun-protected （retroauricular skin） sites of 6 patients with facial photoaging from 2007 to 2008. Genomic microarray analysis was conducted to identify differentially expressed genes between the two groups of specimens followed by gene-cluster analysis. Results The normalization of microarray data showed that the number of differentially expressed genes was 2163 between skin samples from sun-exposed and sun-protected sites in one patient, significantly higher than that in the other 5 patients （less than 200）; therefore, the data from the patient with 2163 differentially expressed genes were excluded from further analysis. Totally, 172 differentially expressed genes were identified with Beadarray chip, including 99 up-regulated genes and 73 down-regulated genes. Based on Genebank research, 118 functionally classified genes were found, which were associated with a series of biological processes, including cell adhesion, receptor regulation, signal transduction, metabolism, and so on. Conclusions There are a lot of differentially expressed genes between human photoaging skin and normal skin. Rhytidectomy may be associated with the differential expression of skin photoaging-related genes.

Key words: Matrix Metalloproteinases

颜薇张利利刘少辉赵振民石兵兵. 应用基因芯片技术筛选皮肤光老化相关基因[J]. 中华皮肤科杂志, 2011, 44(2): 130-133.

参考文献

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